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Identification of new sources of resistance to resistance-breaking isolates of tomato spotted wilt virus
Saudi Journal of Biological Sciences ( IF 4.4 ) Pub Date : 2021-02-21 , DOI: 10.1016/j.sjbs.2021.02.053
Aylin Kabaş , Hakan Fidan , M. Batuhan Demirelli

The tomato as both a fresh consumption and industrial product is one of the most profitable vegetables and has a large cultivation area in the world. Parallel to intense production activities, Tomato Spotted Wilt Virus (TSWV), like viral diseases, results in significant economic losses every year. Use of resistant cultivars is the most efficient and environmental-friendly method of fighting against these diseases. This study was conducted to develop new tomato genetic resources resistant to TSWV because of the Sw-5 resistance breaking (RB) isolates that were determined in tomato cultivation areas. In this study, a total of 40 tomato materials including 15 lines, 9 commercial varieties and 16 wild genotypes were by tested with molecular and biological testing methods. Mechanical inoculation method was used for biological testing and SCAR marker was used in molecular analysis. S. penellii, S. chmielewski, S. habrochaites, S. peruvianum and S. sitiens, LA0716, LA1028, LA1777, LA2744 and LA4110 genotypes were found as resistant against breaking isolates of Tomato Spotted Wilt Virus. These genotypes may be a good resistance source for the future breeding studies in tomato.



中文翻译:

鉴定抗番茄斑萎病毒分离株的新抗性来源

番茄既是新鲜食品又是工业产品,是最赚钱的蔬菜之一,在世界范围内有很大的种植面积。与激烈的生产活动并行,番茄斑萎病毒(TSWV)像病毒性疾病一样,每年都造成巨大的经济损失。使用抗性品种是对抗这些疾病的最有效,最环境友好的方法。进行这项研究是为了开发新的因Sw-5而对TSWV产生抗性的番茄遗传资源在番茄种植区确定的抗性分离株(RB)。在这项研究中,通过分子和生物学测试方法对包括15个品系,9个商业品种和16个野生基因型在内的40种番茄材料进行了测试。机械接种方法用于生物学测试,SCAR标记物用于分子分析。S. penelliiS. chmielewski多毛番茄S. peruvianumS. sitiens,LA0716,LA1028,LA1777,LA2744和LA4110的基因型被发现作为对破番茄株抗斑萎病毒。这些基因型可能是未来番茄育种研究的良好抗性来源。

更新日期:2021-04-30
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