Periodontitis is a condition that occurs because of inflammation-mediated tissue degeneration. Many studies have been conducted to identify inflammatory molecules in periodontitis, but the well-defined role of cells from the immune system in the progression of periodontitis as well as in gingival tissue degeneration has not been appropriately established. The objective of the present study was to characterize the monocytes isolated from the gingival crevicular fluid (GCF) in patients with periodontitis. GCF was obtained from periodontitis patients and healthy controls. Cytokine levels of CCL2 were evaluated by ELISA in GCF samples. CD14+ monocytes were separated using magnetic sorting from GCF. RT-qPCR was performed to assess the gene expression. Cytometric bead array analysis was performed to analyze the levels of cytokines and chemokines in the secretome of cells. CD14+ monocytes from GCF secreted higher levels of CCL2 and showed elevated expression of genes responsible for monocyte migration. Additionally, upon lipopolysaccharide stimulation, these monocytes secreted higher levels of inflammatory cytokines and chemokines. This investigation aids in understanding the inflammatory microenvironment of periodontitis by characterizing GCF in terms of infiltrated CD14+ monocytes, cytokines, and molecules secreted by these monocytes, which are specific for cellular differentiation.

牙周炎是由于炎症介导的组织变性而发生的疾病。已经进行了许多研究来鉴定牙周炎中的炎症分子，但是尚未适当确定免疫系统细胞在牙周炎进展以及牙龈组织变性中的明确作用。本研究的目的是鉴定患有牙周炎的患者从龈沟液（GCF）中分离出的单核细胞。GCF获自牙周炎患者和健康对照。通过ELISA在GCF样品中评估了CCL2的细胞因子水平。使用GCF磁性分选技术分离CD14 +单核细胞。进行RT-qPCR以评估基因表达。进行细胞计数珠阵列分析以分析细胞分泌物中细胞因子和趋化因子的水平。GCF的CD14 +单核细胞分泌更高水平的CCL2，并显示负责单核细胞迁移的基因表达升高。另外，在脂多糖刺激下，这些单核细胞分泌更高水平的炎性细胞因子和趋化因子。这项研究通过根据浸润的CD14 +单核细胞，细胞因子和这些单核细胞分泌的分子（对细胞分化具有特异性）来表征GCF，从而有助于了解牙周炎的炎症微环境。这些单核细胞分泌更高水平的炎症细胞因子和趋化因子。这项研究通过根据浸润的CD14 +单核细胞，细胞因子和这些单核细胞分泌的分子（对细胞分化具有特异性）来表征GCF，从而有助于了解牙周炎的炎症微环境。这些单核细胞分泌更高水平的炎症细胞因子和趋化因子。这项研究通过根据浸润的CD14 +单核细胞，细胞因子和这些单核细胞分泌的分子（对细胞分化具有特异性）来表征GCF，从而有助于了解牙周炎的炎症微环境。