Bisphenol A (BPA), an endocrine disruptor, exist in almost all waters. In the present study, we expose adult male Gobiocypris rarus rare minnow to 15 μg/L BPA to study the effect BPA on fish hepatic lipid metabolism. Following 1, 3 and 5 weeks exposure, the liver tissue of rare minnow was separated. The change of the hepatic morphology, hepatosomatic index, lipid composition and expression of lipid metabolism related genes were analyzed through paraffin section, oil red O staining, lipidomic analysis, and quantitative real-time PCR. BPA can cause significant hepatic lipid deposition in male rare minnow, leading to an increase in triglyceride (TG) level (1.84–22.87-fold), but it is also accompanied by a decrease in diglyceride level (1.67–4.78-fold). The expression of lipid metabolism related genes showed that BPA exposure can up-regulate TG synthesis related genes expression, and down-regulate TG degradation genes expression. Expression of TG transport related genes were also disrupted by BPA. It suggests that BPA can up-regulate rare minnow hepatic TG level through multi-path, and ultimately lead to lipid accumulation in the liver. The results of the present study enrich the mechanisms of environmental endocrine disruptors affecting lipid accumulation in fish.

双酚A（BPA）是一种内分泌干扰物，几乎存在于所有水域。在本研究中，我们暴露了成年雄性Gobiocypris rarus以15μg/ L的双酚A稀少的小鱼，研究双酚A对鱼肝脂质代谢的影响。在暴露1、3和5周后，稀有min鱼的肝脏组织被分离。通过石蜡切片，油红O染色，脂质组分析和定量实时PCR分析肝形态，肝体指数，脂质组成和脂质代谢相关基因表达的变化。BPA可能在雄性稀有min鱼中引起明显的肝脂质沉积，导致甘油三酸酯（TG）水平升高（1.84–22.87倍），但同时伴有甘油二酸酯水平降低（1.67–4.78倍）。脂质代谢相关基因的表达表明，BPA暴露可以上调TG合成相关基因的表达，而下调TG降解基因的表达。TG转运相关基因的表达也被BPA破坏。提示双酚A可以通过多种途径上调罕见的min鱼肝脏TG水平，并最终导致肝脏中脂质的积累。本研究的结果丰富了环境内分泌干扰物影响鱼类脂质积累的机制。