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Simultaneous estimation of four different biomarkers in Cinnamomum verum J. Presl bark using a validated high-performance thin-layer chromatography method
JPC - Journal of Planar Chromatography - Modern TLC ( IF 1.6 ) Pub Date : 2021-02-20 , DOI: 10.1007/s00764-021-00082-z
Shikhar Verma , Jyotsana Dwivedi , Richa Srivastava , Prakash Deep , Vivek Srivastava , Ajay Kumar Singh Rawat , Abhishek Gupta

A high-performance thin-layer chromatography method for the simultaneous quantitative determination of salicylic acid, kaempferol, gallic acid, and protocatechuic acid in hydro-alcoholic fractions of Cinnamomum verum J. Presl bark was developed for the first time in this species. For achieving good separation, a mobile phase of toluene‒ethyl acetate‒formic acid (5:4:1, V/V) was used. Densitometric determination was carried out at specified wavelengths for different standard compounds in reflection/absorption mode. The calibration curves were linear in the range of 100‒600 ng per spot for salicylic acid, kaempferol, gallic acid, and protocatechuic acid. During the analysis, defatted hydro-alcoholic fraction from C. verum J. Presl bark showed the presence of salicylic acid (0.098% w/w), kaempferol (0.24% w/w), gallic acid (0.01% w/w), and protocatechuic acid (0.27% w/w). The proposed method is simple, precise, specific, and accurate. The statistical analysis of the data obtained proves that the method is reproducible and selective and can be used for the routine analysis of the reported phenolic compounds in crude drug and extracts. The simultaneous quantification of these biomarkers has not yet been reported in C. verum J. Presl which may be utilized for the proper standardization of the drug. Research also provides new herbal options for different medicinally valuable secondary metabolites in polar categories with different therapeutic potentials. Exploration of bioactive metabolites in C. verum J. Presl bark can also increase its industrial value, which may promote its cultivation and may create a good commercial option for our farmers.



中文翻译:

使用经过验证的高效薄层色谱方法同时估算肉桂皮中四种不同的生物标志物

一种高效薄层色谱法,用于同时定量测定肉桂肉桂水醇馏分中的水杨酸,山emp酚,没食子酸和原儿茶酸。该物种首次开发了Presl树皮。为了获得良好的分离效果,使用了甲苯‒乙酸乙酯‒甲酸的流动相(5:4:1,V / V)。在指定的波长下,以反射/吸收模式对不同标准化合物进行光密度测定。水杨酸,山ka酚,没食子酸和原儿茶酸的校准曲线在每点100-600 ng范围内呈线性。在分析过程中,提取了淡色梭菌的脱脂乙醇馏分J. Presl树皮显示存在水杨酸(0.098%w / w),山emp酚(0.24%w / w),没食子酸(0.01%w / w)和原儿茶酸(0.27%w / w)。所提出的方法简单,精确,具体且准确。对获得的数据进行统计分析证明,该方法具有可重现性和选择性,可用于常规分析所报道的原料药和提取物中的酚类化合物。这些生物标志物的同时定量尚未在C. verum中报道J. Presl,可用于适当的药物标准化。研究还为具有不同治疗潜力的极地类别中具有药用价值的次生代谢产物提供了新的草药选择。在生物活性代谢产物的探索C.八角J. Presl的树皮也可以增加其产业价值,这可能宣传种植和可能会给我们农民一个很好的商业选择。

更新日期:2021-02-21
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