Hypoxia plays an important role in tumor phenotype and progression and alters glycolysis, with changes in signaling pathways that develop in response to hypoxia. In this study, the effects of oxygen (normoxia/hypoxia) and of glucose levels on the glucose metabolism was investigated in MCF-7 cancer cells. Under either normoxia or hypoxia conditions, the cells were exposed to glucose at different concentrations (0, 5.5, 15 or 55 mM) for either 3, 6, 12, 24 or 48 h. In all groups, cell viability, levels of key enzymes reflecting glycolytic metabolism in cell lysates, glucose consumed in the medium and extracellular lactate levels and wound closure percentages were determined. In hypoxic cells, intracellular consumption of glucose, and extracellular lactate levels due to increased glucose concentration were observed to be higher (compared to normoxia) and as a result of prolonged exposure to hypoxia, cells were observed to develop resistance to the prolonged exposure to hypoxia. The number of glycolytic enzymes obtained at different levels proved that cells had different potential capacities and changing mechanisms for the metabolic needs of the cell depending on the glucose amount in the medium and time in adapting to the oxygen tension. This study showed that there was an important interaction between hypoxia and glucose metabolism in general, and it was concluded that metabolic processes activated by hypoxia could offer new therapeutic targets.

缺氧在肿瘤表型和进展中起重要作用并改变糖酵解，伴随着响应于缺氧而发展的信号通路的变化。在这项研究中，在 MCF-7 癌细胞中研究了氧气（常氧/缺氧）和葡萄糖水平对葡萄糖代谢的影响。在常氧或缺氧条件下，细胞暴露于不同浓度（0、5.5、15 或 55 mM）的葡萄糖 3、6、12、24 或 48 小时。在所有组中，测定了细胞活力、反映细胞裂解物中糖酵解代谢的关键酶水平、培养基中消耗的葡萄糖和细胞外乳酸水平以及伤口闭合百分比。在缺氧细胞中，细胞内消耗葡萄糖，观察到由于葡萄糖浓度增加导致的细胞外乳酸水平更高（与常氧相比），并且由于长时间暴露于低氧环境，观察到细胞对长时间暴露于低氧环境产生抗性。在不同水平获得的糖酵解酶的数量证明，细胞对细胞代谢需求的潜在能力和变化机制取决于培养基中葡萄糖的含量和适应氧张力的时间。该研究表明，总体而言，缺氧和葡萄糖代谢之间存在重要的相互作用，并得出结论，缺氧激活的代谢过程可以提供新的治疗靶点。在不同水平获得的糖酵解酶的数量证明，细胞对细胞代谢需求的潜在能力和变化机制取决于培养基中葡萄糖的含量和适应氧张力的时间。该研究表明，总体而言，缺氧和葡萄糖代谢之间存在重要的相互作用，并得出结论，缺氧激活的代谢过程可以提供新的治疗靶点。在不同水平获得的糖酵解酶的数量证明，细胞对细胞代谢需求的潜在能力和变化机制取决于培养基中葡萄糖的含量和适应氧张力的时间。该研究表明，总体而言，缺氧和葡萄糖代谢之间存在重要的相互作用，并得出结论，缺氧激活的代谢过程可以提供新的治疗靶点。