Long non-coding RNAs recently have been identified as influential indicators in a variety of malignancies. The aim of this study was to identify a functional lncRNA LINC00488 and its effects on thyroid cancer in the view of cell proliferation and apoptosis. In order to evaluate the effects of LINC00488 on the cellular process of thyroid cancer, we performed a series of in vitro experiments, including CCK-8 assay, EdU assay, flow cytometry, transwell chamber assay, western blot and RT-qPCR. The target gene of LINC00488 was then identified by bioinformatics analysis . Finally, a series of recuse experiments were conducted to validate the effect of LINC00488 and its target genes on proliferation, migration, invasion and apoptosis of thyroid cancer. Results: Our findings revealed that LINC00488 was highly expressed in thyroid cancer cell lines (BCPAP, BHP5-16, TPC-1 and CGTH-W3) and promoted the proliferation, migration and invasion, while inhibited the apoptosis of thyroid cancer cells (BCPAP and TPC-1). The results of bioinformatics analysis and dual luciferase reporter gene assay showed that LINC00488 could directly bind to miR-376a-3p and downregulated the expression level of miR-376a-3p. In addition, Paraoxonase-2 (PON2) was a target gene of miR-376a-3p and negatively regulated by miR-376a-3p. Rescue experiment indicated that LINC00488 might enhance PON2 expression by sponging miR-376a-3p in thyroid cancer. our study revealed that lncRNA LINC00488 acted as an oncogenic gene in the progression of thyroid cancer via regulating miR-376a-3p/PON2 axis, which indicated that LINC00488-miR-376a-3p-PON2 axis could serve as novel biomarkers or potential targets for the treatment of thyroid cancer.

中文翻译：

---

长链非编码 RNA LINC00488 通过 miR-376a-3p/PON2 促进甲状腺癌细胞进展。

最近，长链非编码 RNA 已被确定为多种恶性肿瘤的有影响力的指标。本研究的目的是从细胞增殖和凋亡的角度鉴定功能性 lncRNA LINC00488 及其对甲状腺癌的影响。为了评估LINC00488对甲状腺癌细胞过程的影响，我们进行了一系列体外实验，包括CCK-8检测、EdU检测、流式细胞术、Transwell小室检测、蛋白质印迹和RT-qPCR。通过生物信息学分析确定了LINC00488的靶基因。最后，通过一系列的回避实验验证了LINC00488及其靶基因对甲状腺癌增殖、迁移、侵袭和凋亡的影响。结果：我们的研究结果表明，LINC00488 在甲状腺癌细胞系（BCPAP、BHP5-16、TPC-1 和 CGTH-W3）促进增殖、迁移和侵袭，同时抑制甲状腺癌细胞（BCPAP 和 TPC-1）的凋亡。生物信息学分析和双荧光素酶报告基因检测结果表明，LINC00488可以直接结合miR-376a-3p，下调miR-376a-3p的表达水平。此外，对氧磷酶-2 (PON2) 是 miR-376a-3p 的靶基因，受 miR-376a-3p 负调控。救援实验表明，LINC00488可能通过海绵状miR-376a-3p在甲状腺癌中增强PON2的表达。我们的研究表明 lncRNA LINC00488 通过调节 miR-376a-3p/PON2 轴在甲状腺癌的进展中充当致癌基因，这表明 LINC00488-miR-376a-3p-PON2 轴可以作为新的生物标志物或潜在靶点甲状腺癌的治疗。