Giardiasis is one of the most prevalent gastrointestinal diseases in the world. It is caused by Giardia, Giardia lamblia, a common and opportunistic zoonotic parasite. The aim of our work is to find a natural and safe alternative treatment for giardiasis, specifically, to determine if probiotic bacteria (Lactobacillus acidophilus, Bifidobacterium bifidum, and L. helveticus) can contribute to treatment, and act as preventives. Sixty weanling albino mice, Mus musculus, were divided into control and experimental, probiotic-fed groups. We determined infection intensity, and cure and prevention rates of giardiasis through ELISA (enzyme-linked immunosorbent assay) of stool samples and histopathological comparison of intestinal tissue. In experimental groups, there was a significant reduction in infection intensity (P < 0.001) on days 10, 15, and 20, while cure rate reached 87.5%. The control group showed no signs of reduced infection or cure and only the group treated with probiotics prior to infection showed significant prevention rates. In the experimental groups, intestinal changes due to giardiasis appeared seven days post-infection. However, almost all of these changes disappeared by the 25th day. Our results suggest a beneficial and significant effect of probiotics in the prevention and treatment of giardiasis in mice.

中文翻译：

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益生菌对兰氏贾第鞭毛虫引起的小鼠胃肠道疾病的预防作用。

贾第鞭毛虫病是世界上最普遍的胃肠道疾病之一。它是由贾第鞭毛虫引起的，贾第鞭毛虫是一种常见的机会性人畜共患寄生虫。我们工作的目的是为贾第鞭毛虫病找到一种天然且安全的替代疗法，特别是确定益生菌（嗜酸乳杆菌、双歧双歧杆菌和瑞士乳杆菌）是否有助于治疗并起到预防作用。将 60 只断奶白化小鼠（Mus musculus）分为对照组和实验性益生菌喂养组。我们通过粪便样本的ELISA（酶联免疫吸附试验）和肠道组织的组织病理学比较来确定贾第鞭毛虫病的感染强度、治愈率和预防率。在实验组中，第 10、15 和 20 天的感染强度显着降低（P < 0.001），治愈率达到87.5%。对照组没有显示感染或治愈减少的迹象，只有在感染前用益生菌治疗的组显示出显着的预防率。在实验组中，贾第鞭毛虫病引起的肠道变化在感染后 7 天出现。然而，几乎所有这些变化都在第 25 天消失了。我们的研究结果表明，益生菌在预防和治疗小鼠贾第鞭毛虫病方面具有有益且显着的作用。