Liver hepatocellular carcinoma (LIHC) is one of the most frequently occurring primary malignant liver tumors and seriously harms people's health in the world. Methylenetetrahydrofolate dehydrogenase 1-like (MTHFD1L) has been shown to be associated with colon cancer cell proliferation, colony formation and invasion. In the present study, a total of 370 LIHC and 51 normal samples data were downloaded from The Cancer Genome Atlas (TCGA) database. Bioinformatics and immunohistochemistry (IHC) analysis showed that MTHFD1L is highly expressed in liver tumors. Correlation analysis suggested the differences of vital status between high- and low-expression MTHFD1L groups of LIHC. Univariate and multivariate Cox proportional hazards regression were performed to identify the relationship between clinical characteristics and overall survival (OS). In addition, to explore whether MTHFD1L has an effect on the immune infiltration of LIHC. The correlation between MTHFD1L expression and 24 immune cells were analyzed by ImmuneCellAI database. Furthermore, we combined three databases CIBERSORT, TIMER and ImmuneCellAI to do a comprehensive validation and determined that dendritic cells (DCs) resting, macrophage M0 and macrophage M2 closely related to the expression of MTHFD1L. The results showed that MTHFD1L was a potential prognostic biomarker for LIHC, and could help to elucidate that how the immune microenvironment promotes liver cancer development.

中文翻译：

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综合生物信息学分析将 MTHFD1L 鉴定为潜在的生物标志物，并与肝细胞癌的免疫浸润相关。

肝细胞癌（LIHC）是世界上最常见的原发性肝脏恶性肿瘤之一，严重危害人们的健康。亚甲基四氢叶酸脱氢酶 1 样 (MTHFD1L) 已被证明与结肠癌细胞增殖、集落形成和侵袭有关。在本研究中，从癌症基因组图谱（TCGA）数据库中下载了总共 370 个 LIHC 和 51 个正常样本数据。生物信息学和免疫组织化学 (IHC) 分析表明 MTHFD1L 在肝脏肿瘤中高度表达。相关性分析提示LIHC高表达和低表达MTHFD1L组之间生命状态存在差异。进行单变量和多变量 Cox 比例风险回归以确定临床特征和总生存 (OS) 之间的关系。此外，探讨MTHFD1L是否对LIHC的免疫浸润有影响。通过ImmuneCellAI数据库分析MTHFD1L表达与24个免疫细胞的相关性。此外，我们结合CIBERSORT、TIMER和ImmuneCellAI三个数据库进行综合验证，确定树突状细胞（DCs）静息、巨噬细胞M0和巨噬细胞M2与MTHFD1L的表达密切相关。结果表明，MTHFD1L 是 LIHC 的潜在预后生物标志物，有助于阐明免疫微环境如何促进肝癌的发展。TIMER和ImmuneCellAI做了综合验证，确定树突状细胞（DCs）静息、巨噬细胞M0和巨噬细胞M2与MTHFD1L的表达密切相关。结果表明，MTHFD1L 是 LIHC 的潜在预后生物标志物，有助于阐明免疫微环境如何促进肝癌的发展。TIMER和ImmuneCellAI做了综合验证，确定树突状细胞（DCs）静息、巨噬细胞M0和巨噬细胞M2与MTHFD1L的表达密切相关。结果表明，MTHFD1L 是 LIHC 的潜在预后生物标志物，有助于阐明免疫微环境如何促进肝癌的发展。