Background:Physical therapy with whole body vibration (WBV) following compressive spinal cord injury (SCI) in rats restores density of perisomatic synapses, improves body weight support and leads to a better bladder function. The purpose of the study was to determine whether the combined treatmentwith WBV plus erythropoietin (EPO) would further improve motor, sensory and vegetative functions after SCI in rats. Methods:Severe compressive SCI at low thoracic level was followed by a single i.p. injection of 2,5μg (250 IU) human recombinant EPO. Physical therapy with WBV started on 14th day after injury and continued over a 12-week post injury period. Locomotor recovery, sensitivity tests and urinary bladder scores were analysed at 1, 3, 6, 9, and 12 weeks after SCI. The closing morphological measurements included lesion volume and numbers of axons in the preserved perilesional neural tissue bridges (PNTB). Results:Assessment of motor performance sensitivity and bladder function revealed no significant effects of EPO when compared to the control treatments. EPO treatment neither reduced the lesion volume, nor increased the number of axons in PNTB. Conclusions:The combination of WBV + EPO exerts no positive effects on hind limbs motor performance and bladder function after compressive SCI in rats.

中文翻译：

---

促红细胞生成素和全身振动联合治疗脊髓压迫性损伤大鼠的运动、敏感和植物恢复

背景：大鼠脊髓压迫性损伤 (SCI) 后全身振动 (WBV) 的物理治疗可恢复周围突触的密度，改善体重支持并导致更好的膀胱功能。本研究的目的是确定 WBV 加促红细胞生成素 (EPO) 联合治疗是否会进一步改善大鼠 SCI 后的运动、感觉和植物功能。方法：在低胸水平的严重压迫性 SCI 后，单次 ip 注射 2.5μg (250 IU) 人重组 EPO。WBV 物理治疗在受伤后第 14 天开始，并在受伤后持续 12 周。在 SCI 后 1、3、6、9 和 12 周分析运动恢复、敏感性测试和膀胱评分。闭合形态测量包括病变体积和保留的周围神经组织桥 (PNTB) 中的轴突数量。结果：与对照治疗相比，对运动表现敏感性和膀胱功能的评估显示 EPO 没有显着影响。EPO 治疗既没有减少病灶体积，也没有增加 PNTB 中的轴突数量。结论：WBV+EPO联合应用对大鼠脊髓压迫性脊髓损伤后的后肢运动性能和膀胱功能无积极影响。