The resonance Raman spectra of bacterial carotenoids have been employed to identify bacterial strains and their intensity changes as a function of ultraviolet (UV) radiation dose have been used to differentiate between live and dead bacteria. In addition, the resonance-enhanced Raman spectra enabled us to detect bacteria in water at much lower concentrations (∼10⁸ cells/mL) than normally detected spectroscopically. A handheld spectrometer capable of recording resonance Raman spectra in situ was designed, constructed, and was used to record the spectra. In addition to bacteria, the method presented in this paper may also be used to identify fungi, viruses, and plants, in situ, and detect infections within a very short period of time.

细菌类胡萝卜素的共振拉曼光谱已被用于鉴定细菌菌株，并且它们的强度变化作为紫外线 (UV) 辐射剂量的函数已被用于区分活细菌和死细菌。此外，共振增强拉曼光谱使我们能够以比正常光谱检测低得多的浓度（～^{10 8 个}细胞/mL）检测水中的细菌。设计、构建了能够原位记录共振拉曼光谱的手持式光谱仪，并用于记录光谱。除细菌外，本文提出的方法还可用于原位识别真菌、病毒和植物，并在很短的时间内检测感染。