Zebrafish has been established as a classical model for developmental studies, yet in the past years, with the explosion of novel technological methods, the use of zebrafish as a model has expanded. One of the prominent fields that took advantage of zebrafish as a model organism early on is hematopoiesis, the process of blood cell generation from hematopoietic stem and progenitor cells (HSPCs). In zebrafish, HSPCs are born early during development in the aorta–gonad–mesonephros region and then translocate to the caudal hematopoietic tissue, where they expand and finally take residence in the kidney marrow. This journey is tightly regulated at multiple levels from extracellular signals to chromatin. In order to delineate the mechanistic underpinnings of this process, next-generation sequencing techniques could be an important ally. Here, we describe genome-wide approaches that have been undertaken to delineate zebrafish hematopoiesis.

中文翻译：

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“斑马钓鱼”的新工具

斑马鱼已被确立为发育研究的经典模型，但在过去几年中，随着新技术方法的爆炸式增长，斑马鱼作为模型的使用范围有所扩大。早期利用斑马鱼作为模式生物的突出领域之一是造血，即造血干细胞和祖细胞 (HSPC) 产生血细胞的过程。在斑马鱼中，HSPCs 在发育早期在主动脉-性腺-中肾区域出生，然后转移到尾部造血组织，在那里它们扩张并最终驻留在肾骨髓中。这一旅程在从细胞外信号到染色质的多个层面受到严格调控。为了描绘这一过程的机制基础，下一代测序技术可能是一个重要的盟友。这里，