We studied the sequential pathology of peste des petits ruminants (PPR) in Black Bengal goats and analyzed virus distribution in tissues and virus shedding following experimental infection with a Bangladeshi isolate of lineage IV PPR virus (PPRV). The early clinical signs like fever, depression, and ocular and nasal discharges first appeared at 4–7 days post-infection (dpi). Three out of eight inoculated goats died at 13, 15, and 18 dpi, and the rest were killed at different time points from 5 to 18 dpi. Initially, the virus multiplied mostly in the lymphoid organs of the pharyngeal region and caused extensive lymphoid destruction and hemorrhages. This was followed by viremia, massive virus replication in the lungs, and pneumonia along with the appearance of the clinical signs. Subsequently, the virus spread to other organs causing necrotic and hemorrhagic lesions, as well as the virus localized in the upper respiratory, oral and intestinal mucosa resulting in catarrhal, erosive, and ulcerative lesions. On hematological and biochemical investigation progressive leukopenia and hypoproteinemia, a gradual increase of serum metabolites and enzymes associated with liver and kidney damage, and electrolyte imbalance were observed. Seroconversion started at 7 dpi and all the surviving animals had serum antibodies at 14 dpi. Virus shedding was observed in nasal and ocular secretions at 4 dpi and in feces and urine at 14 dpi, which gradually increased and continued till the end of the experiment (18 dpi) despite seroconversion. Therefore, the virus shedding of naturally infected seroconverted goats should be monitored for effective control strategies.

我们研究了孟加拉黑山羊的小反刍兽疫（PPR）的顺序病理学，并分析了孟加拉国IV系PPR病毒（PPRV）的实验性感染后组织中的病毒分布和病毒脱落。早期的临床体征，如发烧，抑郁，以及眼和鼻分泌物，首先出现在感染后（dpi）4-7天。八只接种山羊中有三只分别在13、15和18 dpi时死亡，其余的则在5到18 dpi的不同时间点处死。最初，该病毒主要在咽部的淋巴器官中繁殖，并引起广泛的淋巴破坏和出血。其次是病毒血症，肺中大量病毒复制和肺炎，以及临床症状的出现。随后，病毒传播到其他器官，导致坏死和出血性病变，以及病毒位于上呼吸道，口腔和肠粘膜的局部，导致卡他性，糜烂性和溃疡性病变。在血液和生化研究中，进行性白细胞减少和低蛋白血症，与肝脏和肾脏损害有关的血清代谢产物和酶逐渐增加以及电解质失衡被观察到。血清转化从7 dpi开始，所有存活的动物的血清抗体均为14 dpi。尽管有血清转换，但在4 dpi的鼻和眼分泌物以及在14 dpi的粪便和尿液中观察到病毒脱落，这种病毒逐渐增加并持续到实验结束（18 dpi）。所以，