We suppose that apigenin may inhibit the cellular process of sepsis‐induced lung injury, which is considered to be a major cause of morbidity and mortality, and may improve inflammation and oxidative stress. The aim of this study was to investigate the potential protective effect of apigenin in a rat model of polymicrobial sepsis. Eight groups consisting of a total of 64 female Wistar albino rats were used for this study. Pro‐inflammatory (TNF‐α, IL‐1‐β, IL‐6) and anti‐inflammatory (TGF‐β, IL‐10) cytokine levels were measured with the enzyme‐linked immunosorbent assay technique, oxidant/antioxidants parameters were measured using the spectrophotometric method and Bax and Caspase‐3 immunohistochemical methods. TNF‐α, TGF‐β, IL‐1β, and IL‐6 levels significantly increased in the sepsis‐induced group than in the control groups, while IL‐10 levels decreased. Lipid peroxidase (LPO), an oxidative stress marker, increased, while the antioxidant defense parameters of superoxide dismutase (SOD), catalase (CAT) activities, glutathione (GSH) levels decreased. Although Bax and Caspase‐3 immunoreactivity and H score levels significantly increased in the sepsis group, significant decreases were found in the groups treated with apigenin. In conclusion, we are of the opinion that apigenin treatment improves lung injury by inhibiting oxidative stress and inflammatory cell damage.

中文翻译：

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芹菜素对败血症诱导的大鼠肺细胞凋亡的抗氧化/抗氧化和抗炎作用的研究

我们认为芹菜素可能抑制败血症诱导的肺损伤的细胞过程，这被认为是发病和死亡的主要原因，并且可以改善炎症和氧化应激。这项研究的目的是研究芹菜素在多菌性脓毒症大鼠模型中的潜在保护作用。由总共64只雌性Wistar白化病大鼠组成的八组用于这项研究。使用酶联免疫吸附测定技术测量促炎（TNF-α，IL-1-β，IL-6）和抗炎（TGF-β，IL-10）细胞因子水平，并测定氧化剂/抗氧化剂参数使用分光光度法，Bax和Caspase-3免疫组织化学法。败血症诱导组的TNF-α，TGF-β，IL-1β和IL-6水平显着高于对照组，而IL-10水平下降。脂质过氧化物酶（LPO），一种氧化应激标记物，增加，而超氧化物歧化酶（SOD），过氧化氢酶（CAT）活性，谷胱甘肽（GSH）的抗氧化防御参数降低。尽管Bax和Caspase-3的免疫反应性和败血症组的H评分水平显着升高，芹菜素治疗组的H评分水平显着降低。总之，我们认为芹菜素治疗可通过抑制氧化应激和炎性细胞损伤来改善肺损伤。