We present a quantum dots (QDs)-based lateral flow immunoassay for the determination of pyrimethanil in fruit and vegetable samples. This QDs-based strip immunoassay had a cut-off value of 25 ng/ml, under optimal conditions; linear calibration for pyrimethanil was obtained in the range of 1.9–13.3 ng/ml. The entire sample detection operation could be completed in 30 min. With the strip assay, vegetable and fruit samples spiked with pyrimethanil were extracted and tested, the average recoveries were ranging from 85.6 to 104.3%, with the coefficient of variation below 15%. The results tested from the strip assay were consistent with that obtained from liquid chromatography coupled with tandem mass spectrometry (R² = .98), indicating the developed QDs-based strip assay is a convincing and useful tool.

中文翻译：

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基于量子点的条带免疫分析法的开发，用于检测水果和蔬菜样品中的嘧霉胺

我们提出了一种基于量子点 (QD) 的侧流免疫测定法，用于测定水果和蔬菜样品中的嘧霉胺。在最佳条件下，这种基于 QD 的条带免疫测定的临界值为 25 ng/ml；嘧霉胺的线性校准范围为 1.9–13.3 ng/ml。整个样品检测操作可在30分钟内完成。使用条带法对加标嘧霉胺的蔬菜和水果样品进行提取和检测，平均回收率在 85.6% 到 104.3% 之间，变异系数低于 15%。条带法检测的结果与液相色谱-串联质谱联用的结果一致（R ² = .98)，表明开发的基于 QD 的条带检测是一种令人信服且有用的工具。