Fusarium wilt (FW) disease of cotton, caused by the fungus Fusarium oxysporum f. sp. vasinfectum (Fov), causes severe losses in cotton production worldwide. Though significant advancements have been made in development of FW‐resistant Upland cotton (Gossypium hirsutum) in resistance screening programs, the precise resistance genes and the corresponding molecular mechanisms for resistance to Fov remain unclear. Herein it is reported that Fov7, a gene unlike canonical plant disease‐resistance (R) genes, putatively encoding a GLUTAMATE RECEPTOR‐LIKE (GLR) protein, confers resistance to Fov race 7 in Upland cotton. A single nucleotide polymorphism (SNP) (C/A) in GhGLR4.8, resulting in an amino acid change (L/I), is associated with Fov resistance. A PCR‐based DNA marker (GhGLR4.8^SNP(A/C)) is developed and shown to cosegregate with the Fov resistance. CRISPR/Cas9‐mediated knockout of Fov7 results in cotton lines extremely susceptible to Fov race 7 with a loss of the ability to induce calcium influx in response to total secreted proteins (SEPs) of Fov. Furthermore, coinfiltration of SEPs with GhGLR4.8^A results in a hypersensitive response. This first report of a GLR‐encoding gene that functions as an R gene provides a new insight into plant–pathogen interactions and a new handle to develop cotton cultivars with resistance to Fov race 7.

中文翻译：

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谷氨酸受体样基因中的单核苷酸突变赋予陆地棉中的枯萎病抗性

棉花枯萎病 (FW) 由真菌尖孢镰刀菌 ( Fusarium oxysporum f ) 引起。sp. vasinfectum ( Fov ) 对全球棉花生产造成严重损失。虽然显著进步已经在FW耐陆地棉（开发已取得陆地棉的抗性筛选程序），精确的抗性基因和相应的分子机制，抗Fov的仍不清楚。据报道，Fov7是一种与经典植物抗病 ( R ) 基因不同的基因，推定编码谷氨酸受体样 (GLR) 蛋白，赋予对Fov 的抗性陆地棉第 7 场比赛。GhGLR4.8 中的单核苷酸多态性 (SNP) (C/A)导致氨基酸变化 (L/I)，与Fov抗性相关。开发了基于 PCR 的 DNA 标记 ( GhGLR4.8 ^SNP(A/C) )，并显示与Fov抗性共分离。CRISPR/Cas9 介导的Fov7敲除导致棉花品系对Fov种族 7极其敏感，并且丧失了响应Fov 的总分泌蛋白 (SEP) 诱导钙流入的能力。此外，SEP 与GhGLR4.8 ^{A 的}共渗导致过敏反应。第一份 GLR 编码基因作为R基因的报告提供了对植物-病原体相互作用的新见解，并提供了开发对Fov 7 号品种具有抗性的棉花品种的新方法。