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Investigations on the impact of the introduction of the Aloe vera into the hydrogel matrix on cytotoxic and hydrophilic properties of these systems considered as potential wound dressings
Biomaterials Advances ( IF 7.9 ) Pub Date : 2021-02-19 , DOI: 10.1016/j.msec.2021.111977
S. Kudłacik-Kramarczyk , A. Drabczyk , M. Głąb , D. Alves-Lima , H. Lin , T.E.L. Douglas , S. Kuciel , A. Zagórska , B. Tyliszczak

In the paper, synthesis of chitosan-based hydrogels modified with Aloe vera juice is presented. The novelty of the research was a combination of hydrogel materials with properties beneficial in viewpoint of their use as modern wound dressings and Aloe vera juice supporting the wound healing process. Hydrogels have been obtained via UV radiation. The impact of the amount of the crosslinking agent as well as the introduction of the Aloe vera juice into the hydrogel matrix has been determined. Performed measurements involved analysis of the swelling ability, characteristics of the surface roughness, determining the release profile of Aloe vera and the contact angles of hydrogels. Furthermore, the analysis of the dehydration process of the polymer membrane, investigations on the cytotoxicity of hydrogels via MTT reduction assay and the neutral red uptake assay as well as the studies on the pro-inflammatory activity have also been performed. It was proved that the addition of Aloe vera juice improves the hydrophilic properties of the materials (e.g. contact angle changed from 82.5° to 73.0°). Next, the use of 25% more of the crosslinker resulted even in the increase of the contact angle by 86%. Modified hydrogels showed higher swelling properties even by 15% than unmodified materials. Furthermore, obtained hydrogels show an ability to release Aloe vera – after 5 h approx. 80% of this additive has been released in an acidic environment. Tested materials do not exhibit cytotoxic properties, the addition of Aloe vera results in an improvement of the viability of L929 murine fibroblasts and, importantly, these materials show lower pro-inflammatory activity than the positive control. Performed investigations allow to state that obtained materials show a great application potential.



中文翻译:

研究将芦荟引入水凝胶基质中对这些系统(潜在的伤口敷料)的细胞毒性和亲水性的影响

在本文中,提出了用芦荟汁改性的壳聚糖基水凝胶的合成。该研究的新颖性是将水凝胶材料与具有多种特性的组合在一起,这些材料在用作现代伤口敷料和支持伤口愈合过程的芦荟汁方面具有优势。水凝胶已经通过紫外线辐射获得。已经确定了交联剂的量以及将芦荟汁引入水凝胶基质中的影响。进行的测量涉及对溶胀能力的分析,表面粗糙度的特征,确定芦荟的释放曲线和水凝胶的接触角。此外,还进行了聚合物膜脱水过程的分析,通过MTT还原测定和中性红吸收测定对水凝胶的细胞毒性的研究以及对促炎活性的研究。事实证明,添加芦荟汁可以改善材料的亲水性(例如,接触角从82.5°变为73.0°)。接下来,甚至使用更多25%的交联剂,甚至导致接触角增加86%。改性的水凝胶显示出比未改性的材料更高的溶胀性能,甚至高出15%。此外,获得的水凝胶显示出释放芦荟的能力–约5小时后 该添加剂的80%已在酸性环境中释放。被测物质不具有细胞毒性,芦荟的添加可提高L929鼠成纤维细胞的活力,重要的是,这些物质的促炎活性低于阳性对照。进行的调查表明,所获得的材料具有巨大的应用潜力。

更新日期:2021-02-24
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