Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions. The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion. Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion. A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups. These results demonstrated that N,O-CS/ODA hydrogel could effectively prevent post-operative peritoneal adhesion without side effects. Therefore, the N,O-CS/ODA hydrogels with multi-functional properties exhibit great potential for the prevention and treatment of postoperative adhesion.

本文中，我们制备了由N，O组成的新型自修复，原位注射，可生物降解和无毒的水凝胶抗粘连屏障材料-羧甲基壳聚糖（N，O-CS）和氧化葡聚糖（ODA）不需要任何化学交联剂或外部刺激触发器即可预防和治疗术后腹膜粘连。N，O-CS / ODA水凝胶具有良好的适宜胶凝时间，良好的细胞相容性和血液相容性，良好的抗菌活性，优异的生物降解性和生物相容性，可以有效抑制成纤维细胞对伤口的粘附，从而提示N，O-CS / ODA水凝胶适用于预防术后粘连。同时，建立了大鼠损伤侧壁-盲肠磨损模型，以研究这些水凝胶在实现术后抗粘连方面的功效。在N区，腹膜粘连明显减少（10％大鼠的评分较低）。O-CS / ODA-水凝胶治疗组与市售水凝胶和对照组相比。这些结果表明，N，O-CS / ODA水凝胶可以有效地防止术后腹膜粘连，而没有副作用。因此，具有多功能特性的N，O-CS / ODA水凝胶具有预防和治疗术后粘连的巨大潜力。