Pteris aspericaulis var. tricolor T. Moore ex Lowe is a fern species with colorful fronds, potentially used as an ornamental plant. In the present study, we reported the establishment of in vitro regeneration of P. aspericaulis var. tricolor via green globular bodies (GGBs) system by using in vitro juvenile sporophytes as explants. GGB induction, multiplication and plantlet regeneration media, as well as the influence of light-emitting diodes (LEDs) on GGB multiplication and plantlet regeneration were investigated. The highest GGB induction frequency obtained on the medium supplemented with 2.73 μM thidiazuron (TDZ). The medium containing 1.32 μM 6-Benzyladenine (BA) was the optimum for GGB multiplication. GGB cultivated on 1/4 MS medium with the addition of 0.1% (w/v) activated charcoal (AC) behaved the highest differentiation frequency (94.44%), and 1/4 MS medium without AC was the most effective for plantlet growth. The light quality of LEDs (white, blue, red) had no effect on GGB multiplication and differentiation, but remarkably affected the plantlet height. Red LEDs promoted the petiole elongation, while blue LEDs inhibited it. Morphological and histological observation showed that GGBs consisted of a cluster of single GGBs with meristem and scale-like structure, and new single GGBs were efficiently initiated from the epidermal cells in top meristem of GGBs.

凤尾aspericaulis变种。Tricolor T. Moore ex Lowe是一种蕨类植物，具有五颜六色的叶状体，可能用作观赏植物。在目前的研究中，我们报道了P. aspericaulis var的体外再生。通过体外使用绿色球状体（GGB）系统生成三色幼孢子体作为外植体。研究了GGB的诱导，繁殖和幼苗再生培养基，以及发光二极管（LED）对GGB繁殖和幼苗再生的影响。在添加2.73μM噻唑隆（TDZ）的培养基上获得的最高GGB诱导频率。含有1.32μM6-苄基腺嘌呤（BA）的培养基是GGB繁殖的最佳选择。GGB在1/4 MS培养基上培养，添加0.1％（w / v）活性炭（AC）表现出最高的分化频率（94.44％），而无AC的1/4 MS培养基对幼苗生长最为有效。LED的光质量（白色，蓝色，红色）对GGB的繁殖和分化没有影响，但显着影响幼苗的高度。红色LED促进叶柄伸长，而蓝色LED抑制叶柄伸长。形态和组织学观察显示，GGB由具有分生组织和鳞片状结构的单个GGB簇组成，并且从GGB的顶部分生组织中的表皮细胞有效地引发了新的单个GGB。