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Screening of oat varieties and landraces at early vegetative stage under salt stress conditions: Morpho-physiological and PCA biplot analysis
Cereal Research Communications ( IF 1.6 ) Pub Date : 2021-02-18 , DOI: 10.1007/s42976-021-00138-0
Hüseyin Güngör , Yakup Çıkılı , Ziya Dumlupınar

Salinity limits the oat germination and seedling growth at early growth stages. This study was aimed to determine the morpho-physiological response of 33 (10 commercial and 23 landraces) oat genotypes to four NaCl salinity stresses (0, 30, 60 and 90 mM) under in vitro conditions during seed germination and early seedling stages. The effect of NaCl stress on water uptake, germination ratio, root length, shoot length, root fresh weight, root dry weight, shoot fresh weight, shoot dry weight, ion contents (Na+, K+, Ca and K+/Na+) for both root and shoot and photosynthetic pigments (Chl a, Chl b, Chl a + b and carotenoid) in 33 oat genotypes was investigated in the experiment. The investigated traits were decreased by salt stress from control (0 mM) to 90 mM doses including 18% for water uptake, 54.9% for germination rate, 87.5% for root length, 87% for shoot length, 74.5% for root fresh weight, 38.5% for root dry weight, 79.1% for shoot fresh weight, 62.4% for shoot dry weight, 84.5% for root K+/Na+, 97% for shoot K+/Na+, 62.2% for chl a, 51.6% for chl b and 61.7% for carotenoid. It was found that the oat genotypes showed a wide variation in the cluster analysis based on morpho-physiological traits. According to the principal component biplot analysis, principal components (PC1 and PC2) represented (33.9 and 16.4%, respectively) 50.3% of the total variation among the genotypes under salt stress. TL582 landrace showed greater performance for the investigated traits among oat genotypes for the highest salt tolerance. The investigated traits are important growth indices and physiological parameters to be used as selection criteria to evaluate salt tolerance in oat genotypes. The results of the study determined the registered and local genotypes that can be used as parent lines in breeding programs.



中文翻译:

盐胁迫条件下营养早期的燕麦品种和地方品种的筛选:形态生理和PCA双图分析

盐度限制了燕麦在早期生长阶段的发芽和幼苗生长。这项研究旨在确定33种(10种商业品种和23种地方品种)燕麦基因型在种子发芽和幼苗早期的体外条件下对四种NaCl盐度胁迫(0、30、60和90 mM)的形态生理响应。NaCl胁迫对水分吸收,发芽率,根长,枝长,根鲜重,根干重,芽鲜重,茎干重,离子含量(Na +,K +,Ca和K + / Na +)的影响)用于根和芽以及光合色素(Chl a,Chl b,Chl a  +  b实验中对33种燕麦基因型进行了研究。盐胁迫从对照(0 mM)到90 mM剂量,使所研究的性状降低,包括摄水量18%,发芽率54.9%,根长87.5%,枝长87%,根鲜重74.5%,根干重38.5%,枝条鲜重79.1%,枝干重62.4%,根K + / Na + 84.5 %,枝条K + / Na + 97 %,chl a 62.2% 51.6% chl b类胡萝卜素占61.7%。根据形态生理特征,在聚类分析中发现燕麦基因型差异很大。根据主成分双图分析,在盐胁迫下,主成分(PC1和PC2)占基因型总变异的50.3%(分别为33.9和16.4%)。TL582地方品种在燕麦基因型中表现出更高的耐盐性表现。研究的性状是重要的生长指标和生理参数,可作为选择标准来评估燕麦基因型的耐盐性。研究结果确定了可以在育种计划中用作亲本的注册基因型和本地基因型。

更新日期:2021-02-19
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