Diploid hybrid potato variety development requires the introduction of reliably transmitted self-compatibility (SC) to largely self-incompatible elite diploid germplasm. The diploid Solanum chacoense clone M6 has been widely used to introgress SC into North American potato diploid breeding programs. We determined that M6 is homozygous for six DNA Kompetitive Allele Specific PCR (KASP)™ markers spanning a 224 kb region, linked to Sli in Dutch germplasm. Self-compatible Sli alleles were identified in dihaploids of the cultivars ‘Atlantic’ and ‘Superior’ and breeding clone NY148. This finding demonstrates the potential of Sli genotyping to select S. tuberosum dihaploids that will contribute to SC. We appraised the transmission of Sli in a diploid recurrent selection population and in a diploid backcross population, each designed to introgress SC while improving agronomic traits. The frequency of the homozygous self-compatible Sli genotype at the six marker loci increased over the course of four cycles of recurrent selection. The homozygous Sli self-compatible genotype at any one of five marker loci within an 80.8 kb region on chromosome 12 (58,960,090-59,040,898 bp) perfectly predicted a SC phenotype in the recurrent selection population. The heterozygous Sli genotype was found in self-compatible and self-incompatible individuals. The discrepancy between phenotype and marker genotype can be attributed in part to the difficulty of accurately phenotyping SC. We also identified self-compatible individuals with the homozygous self-incompatible Sli genotype at all tested loci. The presence of the homozygous self-incompatible Sli haplotype in self-compatible clones 1S1 and DMRH-89 and a self-compatible individual from the S. chacoense PI 133664 further suggests that other genetic components contribute to SC. This work illustrates the ability of Sli markers to predict SC in some germplasm, but it also underscores the need to identify other genomic regions critical to SC and the role of the environment in expression of genes involved in the SC reaction.

二倍体杂交马铃薯品种的开发需要将可靠传播的自我相容性（SC）引入到很大程度上自我不相容的优良二倍体种质中。二倍体茄果克隆M6已被广泛用于将SC渗入北美马铃薯二倍体育种程序。我们确定M6对跨越荷兰种质的Sli的六个224 kb区域的DNA竞争性等位基因特异性PCR（KASP）™标记是纯合的。在品种'Atlantic'和'Superior'的二倍体和繁殖克隆NY148中鉴定出了自相容的Sli等位基因。这一发现证明了Sli基因分型的选择潜力。将有助于SC的二倍体。我们评估了Sli在二倍体轮回选择群体和二倍体回交群体中的传播，它们各自旨在使SC渗入，同时改善农艺性状。在四个循环选择过程中，六个标记基因座的纯合子自相容Sli基因型的频率增加。在12号染色体上的80.8 kb区域（58,960,090-59,040,898 bp）中的五个标记位点中的任何一个上，纯合的Sli自相容基因型完美地预测了复发选择人群中的SC表型。杂合子Sli在自我相容和自我不相容的个体中发现了基因型。表型和标记基因型之间的差异可部分归因于准确进行SC表型分析的困难。我们还确定了在所有测试位点具有纯合的自我不相容的Sli基因型的自我相容的个体。纯合的自交不亲和的存在的Sli在自交亲克隆1S1和DMRH-89，并从自个体兼容单倍型S. chacoense PI 133664还建议其他遗传成分到SC贡献。这项工作说明了Sli的能力 标记可以预测某些种质中的SC，但也强调需要确定对SC至关重要的其他基因组区域，以及环境在涉及SC反应的基因表达中的作用。