Brillouin light scattering offers a unique label-free approach to measure biomechanical properties non-invasively. While this technique is used in biomechanical analysis of cells and tissues, its potential for visualizing structural features of tissues based on the biomechanical contrast has not been much exploited. Here, we present high-resolution Brillouin microscopy images of four basic tissue types: muscular, connective, epithelial, and nervous tissues. The Brillouin contrast distinguishes between muscle fiber cells and endomysium in skeletal muscle and reveals chondrocytes along with spatially varying stiffness of the extracellular matrix in articular cartilage. The hydration-sensitive contrast can visualize the stratum corneum, epidermis, and dermis in the skin epithelium. In brain tissues, the Brillouin images show the mechanical heterogeneity across the cortex and deeper regions. This work demonstrates the versatility of using the Brillouin shift as histological contrast for examining intact tissue substructures via longitudinal modulus without the need for laborious tissue processing steps.

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使用布里渊光散射对比的组织无标记组织学成像

布里渊光散射提供了一种独特的无标记方法来非侵入性地测量生物力学特性。虽然该技术用于细胞和组织的生物力学分析，但其基于生物力学对比可视化组织结构特征的潜力尚未得到充分利用。在这里，我们展示了四种基本组织类型的高分辨率布里渊显微镜图像：肌肉组织、结缔组织、上皮组织和神经组织。布里渊对比区分骨骼肌中的肌纤维细胞和肌内膜，并揭示软骨细胞以及关节软骨中细胞外基质的空间变化刚度。水化敏感对比可以显示皮肤上皮中的角质层、表皮和真皮。在脑组织中，布里渊图像显示了整个皮层和更深区域的机械异质性。这项工作证明了使用布里渊位移作为组织学对比的多功能性，用于通过纵向模量检查完整的组织亚结构，而无需费力的组织处理步骤。