Background: Post-contrast acute kidney injury (PC-AKI) is a severe complication of cardiac catheterization. Emerging evidence indicated that long non-coding RNAs (lncRNAs) could serve as biomarkers for various diseases. However, the lncRNA expression profile and potential biomarkers in PC-AKI remain unclear. This study aimed to investigate novel lncRNA biomarkers for the early detection of PC-AKI./nMethods: lncRNA profile in the kidney tissues of PC-AKI rats was evaluated through RNA sequencing. Potential lncRNA biomarkers were identified through human-rat homology analysis, kidney and blood filtering in rats and verified in 112 clinical samples. The expression patterns of the candidate lncRNAs were detected in HK-2 cells and rat models to evaluate their potential for early detection./nResults: In total, 357 lncRNAs were found to be differentially expressed in PC-AKI. We identified lnc-HILPDA and lnc-PRND were conservative and remarkably upregulated in both kidneys and blood from rats and the blood of PC-AKI patients; these lncRNAs can precisely distinguish PC-AKI patients (area under the curve (AUC) values of 0.885 and 0.875, respectively). The combination of these two lncRNAs exhibited improved accuracy for predicting PC-AKI, with 100% sensitivity and 83.93% specificity. Time-course experiments showed that the significant difference was first noted in the blood of PC-AKI rats at 12 h for lnc-HILPDA and 24 h for lnc-PRND./nConclusion: Our study revealed that lnc-HILPDA and lnc-PRND may serve as the novel biomarkers for early detection and profoundly affect the clinical stratification and strategy guidance of PC-AKI.

中文翻译：

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从长的非编码 RNA 表达谱中鉴定出用于对比后急性肾损伤的新型生物标志物

背景：增强后急性肾损伤 (PC-AKI) 是心导管插入术的严重并发症。新出现的证据表明，长链非编码 RNA (lncRNA) 可以作为各种疾病的生物标志物。然而，PC-AKI 中的 lncRNA 表达谱和潜在的生物标志物仍不清楚。本研究旨在研究用于早期检测 PC-AKI 的新型 lncRNA 生物标志物。/n方法：通过 RNA 测序评估 PC-AKI 大鼠肾脏组织中的 lncRNA 谱。通过人鼠同源性分析、大鼠肾脏和血液过滤鉴定了潜在的 lncRNA 生物标志物，并在 112 个临床样本中进行了验证。在 HK-2 细胞和大鼠模型中检测候选 lncRNA 的表达模式，以评估它们的早期检测潜力。/n结果：总共发现 357 个 lncRNA 在 PC-AKI 中存在差异表达。我们发现 lnc-HILPDA 和 lnc-PRND 在大鼠的肾脏和血液以及 PC-AKI 患者的血液中是保守的并且显着上调；这些 lncRNA 可以精确区分 PC-AKI 患者（曲线下面积 (AUC) 值分别为 0.885 和 0.875）。这两种 lncRNA 的组合在预测 PC-AKI 方面表现出更高的准确性，灵敏度为 100%，特异性为 83.93%。时程实验表明，lnc-HILPDA 12 h 和 lnc-PRND 24 h 时 PC-AKI 大鼠血液中首次出现显着差异。/n结论： 我们的研究表明，lnc-HILPDA 和 lnc-PRND 可作为早期检测的新型生物标志物，并深刻影响 PC-AKI 的临床分层和策略指导。