ABSTRACT

The Mediterranean fruit fly,Ceratitis capitata (Diptera: Tephritidae), is an important economic pest worldwide causing direct crop damage. One of the most successful control strategies for this pest is the application of the sterile insect technique. However, the deterioration of competitive mating performance against wild males was observed in Vienna8 Genetic Sexing Strain sterile males, which was aconsequence of irradiation. Therefore, beneficial probiotics were investigated to use as supplementation in sterile insect diets with properties on sexual performance enhancing which was the objective of the study. Hence, bacterial strains were isolated from C. capitata wild strain guts and were identified using the 16S-23S rRNA internal transcribed spacers region and 16S rRNA sequencing method. The isolated strains were evaluated for their probiotic potential by ‘in vitro’ and ‘in vivo’ tests. Finally, the candidate probiotic strains were selected by statistical analysis of their ‘in vitro’ and ‘in vivo’ tests data using principal component analysis (PCA). In conclusion, the combination of the two PCAs allowed the selection of Enterobacter sp., Lactococcus lactis and Klebsiella oxytoca as potential probiotic candidates to integrate into the larval diet.

摘要

地中海果蝇ceratitis capitata  （Diptera：Tephritidae）是一种重要的经济害虫，在世界范围内造成直接的作物损害。该害虫最成功的防治策略之一是应用无菌昆虫技术。然而，在Vienna8遗传性别不育雄性中观察到了与野生雄性竞争交配性能的下降，这是辐射的结果。因此，研究了有益的益生菌，以在无菌昆虫饮食中用作补充品，具有增强性能力的特性，这是本研究的目的。因此，从C. capitata分离出细菌菌株 。 野生菌株的肠道，并使用16S-23S rRNA内部转录间隔区和16S rRNA测序方法进行鉴定。通过“体外”和“体内”测试评估分离的菌株的益生菌潜力。最后，使用主成分分析（PCA）通过统计分析其“体外”和“体内”测试数据来选择候选益生菌菌株。总之，两种PCA的组合允许选择 肠杆菌 属， 乳酸乳球菌 和产 酸克雷伯菌 作为潜在的益生菌候选物并入幼虫饮食。