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Novel potential yeast strains for the biotechnological production of xylitol from sugarcane bagasse
Biofuels, Bioproducts and Biorefining ( IF 3.9 ) Pub Date : 2021-02-18 , DOI: 10.1002/bbb.2196
Fernanda Palladino 1 , Rita C L B Rodrigues 2 , Raquel M Cadete 1 , Katharina O Barros 1 , Carlos A Rosa 1
Affiliation  

This study aimed to screen the xylitol‐producing strains of Cyberlindnera xylosilytica and Wickerhamomyces rabaulensis for the bioconversion of the hemicellulosic fraction of sugarcane bagasse. Among the 16 strains tested, two of each species (C. xylosilytica UFMG‐CM‐Y309 and UFMG‐CM‐Y409, and W. rabaulensis UFMG‐CM‐Y3716 and UFMG‐CM‐Y3747) were selected on the basis of xylitol yield (Yp/s) and xylitol volumetric productivity (Qp) in YPX broth (yeast extract, 10 g L−1; peptone, 20 g L−1; and d‐xylose, 60 g L−1). These strains were cultivated in sugarcane bagasse hemicellulosic hydrolysate (SCHH) supplemented with ammonium sulfate, rice bran extract, and yeast extract. The xylitol yield was the highest in the strain C. xylosilytica UFMG‐CM‐Y‐309 with a production of 14.06 g L−1; xylitol yield, 0.63 g g−1; xylitol volumetric productivity, 0.20 g L−1 h−1. Xylitol yields ranged from 14% to 74% above those of other xylitol‐production yeast species. This work demonstrates the potential of biotechnological application of these yeasts for xylitol production. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

中文翻译:

从甘蔗渣中生物技术生产木糖醇的新型潜在酵母菌株

这项研究旨在筛选产木糖醇的Cyber​​lin德拉xylosilyticaWickerhamomyces rabaulensis菌株,以对甘蔗渣的半纤维素部分进行生物转化。在测试的16个菌株中,根据木糖醇产量选择了两个物种(木糖衣线虫UFMG‐CM‐Y309和UFMG‐CM‐Y409,以及拉波乌尔氏菌UFMG‐CM‐Y3716和UFMG‐CM‐Y3747)。 (Y p / s)和YPX肉汤(酵母提取物,10 g L -1;蛋白one,20 g L -1;和d-木糖,60 g L -1)中的木糖醇容积生产率(Q p)。这些菌株在补充有硫酸铵,米糠提取物和酵母提取物的甘蔗渣半纤维素水解产物(SCHH)中培养。木糖醇收率在C.xylosilytica UFMG-CM-Y-309菌株中最高,产量为14.06 g L -1。木糖醇产率,0.63g g -1;木糖醇的容积生产率为0.20 g L -1  h -1。木糖醇的产量比其他木糖醇生产酵母种类高14%至74%。这项工作证明了这些酵母在木糖醇生产中的生物技术应用潜力。©2021化学工业协会和John Wiley&Sons,Ltd
更新日期:2021-02-18
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