It has been recently confirmed that photoexcited ketones such as acetone promote the chemiluminescence (CL) reaction of luminol and its analog, L-012. Using this finding, a selective determination method for acetone was developed herein based on HPLC separation, online UV irradiation, and subsequent luminol CL detection. Various conditions, including the type of luminol reagent, the use of organic solvents as eluent modifiers, and the reaction pH, were investigated to elucidate the CL reaction mechanism. The results showed that the CL reaction of luminol is caused by an acetone ketyl radical produced from the photoexcited acetone. The performance of the novel acetone determination method was evaluated by its application in water quality assurance in water treatment plants. The method detection limit and the method quantification limit of acetone were 0.03 and 0.08 µM, respectively. A linear range was obtained in the concentration range of 0.1–200 µM. The range of recovery in the wastewater and seawater samples (n = 5) fortified with 5.0 µM acetone was 87–108%. These results demonstrate that the new luminol CL system is useful for its sensitivity and selectivity.

最近已经证实，光激发的酮例如丙酮促进鲁米诺及其类似物L-012的化学发光（CL）反应。利用这一发现，本文开发了一种基于HPLC分离，在线UV辐照和随后的鲁米诺CL检测的丙酮选择性测定方法。研究了各种条件，包括鲁米诺试剂的类型，使用有机溶剂作为洗脱剂改性剂以及反应pH值，以阐明CL反应机理。结果表明，鲁米诺的CL反应是由光激发丙酮产生的丙酮酮基自由基引起的。通过其在水处理厂水质保证中的应用，评估了该新型丙酮测定方法的性能。丙酮的方法检测限和方法定量限分别为0.03和0.08 µM。在0.1–200 µM的浓度范围内获得线性范围。废水和海水样品的回收范围（n  = 5）用5.0 µM丙酮强化的含量为87–108％。这些结果表明，新的鲁米诺CL系统对于其灵敏度和选择性很有用。