This research study is mainly focused to evaluate the anti-parasitic, insecticidal, cytotoxic and anti-alzheimer potential of various leaf extracts of Ajuga bracteosa Wallich ex Bentham. 04 different extracts were prepared using solvent of different polarity to determine the best candidate for potent bioactivity i.e. n-hexane (NH), Ethyl acetate (EA), Ethanol (EL) and Chloroform (CH). Concentrations of each extracts were made specified for all activities. All extracts were exploited for broad range of biomedical applications including leishmaniasis, in vitro anti-Alzheimer, insecticidal and cytotoxic studies. Our results showed that A. bracteosa n-hexane extract was highly active against Leishmania Tropica with significant inhibition of 58 ± 1.61 for promastigote and 63 ± 2.29 for amastigote at 1000 μg/mL. Furthermore, promising anti-alzheimer activity acetylcholinesterase (AChE) 46 ± 0.83 and butrylcholineterase (BChE) 49 ± 1.17 was noted for n-hexane. The insecticidal potential of these extracts were test against five different insects (Rhyzopertha dominica, Trogoderma granarium, Tribolium castaneum, Sitophilus oryze, and Callosobruchus analis). The higest mortality rate of insecticidal activity was recorded by n-hexane followed by Ethyl acetate whereas ethanol extract was found to be less effective against all the test species. Significant cytotoxic potential of each plant sample against Artemia salina thus aware us for further detailed research to find out novel drugs. Based on our results we believe that Ajuga bracteosa could be used to develop as a potential botanical insecticide against different insect and pests, such as aphids as well as an excellent source for the compound isolation as anti-tumor agent.

本研究主要旨在评估Ajuga bracteosa Wallich ex Bentham各种叶提取物的抗寄生虫、杀虫、细胞毒性和抗阿尔茨海默病潜力。使用不同极性的溶剂制备 04 种不同的提取物，以确定具有有效生物活性的最佳候选物，即正己烷 (NH)、乙酸乙酯 (EA)、乙醇 (EL) 和氯仿 (CH)。每种提取物的浓度均针对所有活动进行了指定。所有提取物均被用于广泛的生物医学应用，包括利什曼病、体外抗阿尔茨海默病、杀虫和细胞毒性研究。我们的结果表明，A. bracteosa 正己烷提取物对热带利什曼原虫具有高度活性，在 1000 μg/mL 时对前鞭毛体和无鞭毛体具有显着抑制作用，分别为 58 ± 1.61 和 63 ± 2.29。此外，正己烷的乙酰胆碱酯酶 (AChE) 46 ± 0.83 和丁酰胆碱酯酶 (BChE) 49 ± 1.17 具有良好的抗阿尔茨海默病活性。这些提取物的杀虫潜力针对五种不同的昆虫（Rhyzopertha dominica、Trogoderma granarium、Tribolium castaneum、Sitophilus oryze和Callosobruchus analis）进行了测试。正己烷的杀虫活性死亡率最高，其次是乙酸乙酯，而乙醇提取物对所有测试物种的效果较差。每种植物样品对卤虫都有显着的细胞毒性潜力，因此我们需要进行进一步的详细研究，以找出新的药物。根据我们的结果，我们相信Ajuga bracteosa可用于开发作为针对不同昆虫和害虫（例如蚜虫）的潜在植物杀虫剂，以及作为抗肿瘤剂的化合物分离的极好来源。