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Development of alternative and sustainable methodologies in laboratory research on sea urchin gametes
Marine Environmental Research ( IF 3.3 ) Pub Date : 2021-02-18 , DOI: 10.1016/j.marenvres.2021.105282
Adele Fabbrocini , Fausto Silvestri , Raffaele D'Adamo

The aim of the present work is to develop a laboratory-scaled methodology for an on-demand supply of semen from the sea urchin Paracentrotus lividus. Firstly, sea urchin specimens were acclimatized to the long-term rearing in a recirculating aquaculture system and gonad maturation was obtained under controlled conditions. Semen samples were then collected from mature sea urchins and cryopreserved. Finally, post-thawing motility was evaluated, to verify whether the cryopreserved semen had maintained enough viability to be used in laboratory activities. The post-thawing motility parameters remained quite unchanged for up to 60 min after activation; moreover, the semen even retained the ability of motility activation for 60 min after thawing. This motility pattern makes the use of cryopreserved semen a feasible option in spermiotoxicity bioassays. The preliminary ecotoxicity test, carried out using motility parameters as endpoints, showed sensitivity levels to cadmium falling in the same order of magnitude as those recorded for fresh sea urchin semen and for cryopreserved sea bream semen. . Therefore, semen samples produced and stored according to the developed methodology described in this paper, can be considered a promising and sustainable alternative to those collected from mature sea urchins harvested in the field.



中文翻译:

海胆配子实验室研究中替代和可持续方法的发展

本工作的目的是为海胆Paracentrotus lividus的精液按需供应开发一种实验室规模的方法首先,将海胆标本适应循环养殖系统中的长期饲养,并在受控条件下获得性腺成熟。然后从成熟的海胆中收集精液样品并冷冻保存。最后,评估解冻后的运动能力,以验证冷冻保存的精液是否保持了足够的活力以用于实验室活动。激活后长达60分钟,解冻后的运动参数仍然保持不变。此外,解冻后精液甚至保留了60分钟的活力激活能力。这种运动模式使冷冻保存的精液在精子毒性生物测定中成为可行的选择。以运动参数为终点进行的初步生态毒性测试,结果显示,对镉的敏感度下降幅度与新鲜海胆精液和冷冻鲷鱼精液记录的数量级相同。。因此,根据本文所述发达方法生产和储存的精液样品,可以认为是从田间收获的成熟海胆采集的精液样品的一种有前途且可持续的选择。

更新日期:2021-02-24
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