The ATP-binding cassette (ABC) proteins are involved in trans-cellular transport of substances at inverse concentrations. After ABC genes were discovered to be involved in multidrug resistance in humans, increased attention was paid to the role of insect ABC genes in pesticide resistance. Chilo suppressalis, the most important Lepidopteran pest in rice production, has developed resistance to the insecticide chlorantraniliprole. However, few of the ABC genes that contribute to chlorantraniliprole resistance have been studied. Here, 47 genes encoding ABC transporters were identified, including five CsABCA, seven CsABCB, ten CsABCC, two CsABCD, one CsABCE, three CsABCF, 15 CsABCG, and four CsABCH genes. The expression profiles of these transporter genes were determined in three chlorantraniliprole-resistant strains: R1, R2, and HR, with a resistance ratio of 38.8, 110.4, and 249.6, respectively. CsABCA1, -D2, and -H2 were up-regulated in all resistant strains, and the trend in the transcript levels of all 47 CsABC genes fitted a descending curve from R1 to HR. Unexpectedly, CsABCF1, -G11 and -G12 were significantly down-regulated in HR. These results provide new insights into the roles of ABC transporters in chlorantraniliprole resistance in C. suppressalis.

ATP结合盒（ABC）蛋白以相反的浓度参与物质的跨细胞运输。在发现ABC基因与人的多药耐药性有关后，人们越来越关注昆虫ABC基因在农药抗性中的作用。在水稻生产中最重要的鳞翅目害虫Chilo inhibitoralis对杀虫剂氯antlaniliprole产生了抗药性。然而，几乎没有研究到能引起对菜豆腈抗性的ABC基因。在这里，鉴定出47个编码ABC转运蛋白的基因，包括5个CsABCA，7个CsABCB，10个CsABCC，2个CsABCD和1个CsABCE，三个CsABCF，15个CsABCG和四个CsABCH基因。这些转运蛋白基因的表达谱是在三种对氯虫腈具有抗性的菌株：R1，R2和HR中确定的，其抗性比分别为38.8、110.4和249.6。CsABCA1，-D2，和-H2表达上调在所有抗性菌株，并在所有47个的转录物水平的趋势CsABC基因嵌合的下降曲线从R1到HR。出乎意料的是CsABCF1，-G11和-G12在人力资源中显着下调。这些结果提供了新的见解，发挥了ABC转运蛋白在抑制隐孢子虫对氯氰胺的抗性中的作用。