Preventive measures based in the control of insect vectors are considered as the best choice to decrease the incidence of insect-borne diseases. Herein we report on the volatile content of the leaf essential oils from Marina neglecta, a medicinal plant distributed in the tropical regions of southern Mexico. In order to investigate the chemical variation of the essential oils, a volatile screening was performed during the four seasons of the years 2016–2019. Simultaneously, their biological activity was tested on distinct life stages of Meccus pallidipennis, M. bassolsae, Aedes aegypti and A. albopictus. Essential oils were mainly constituted of β-pinene (>30%) β-caryophyllene (>25%) and germacrene D (>13%). Dorsal-abdomen application of essential oils on triatomines, revealed an efficient LC₅₀ for nymphs of the stages I to III (4 µg/insect), nymphs of the stages IV to V (5–6 µg/insect), and adults (7–8 µg/insect). The LT₅₀ for the stages I to III was between 6 and 8 h, whereas that for the stages IV to V and adults oscillated between 12 and 16 h and 22 to 26 h, respectively. Fumigation experiments performed on nymph V, demonstrated that 300 µg L⁻¹ air produced 100% mortality after 72 h post-treatment. Among tested volatiles, β-pinene and β-caryophyllene produced a comparable mortality rate (p < 0.01) than that of essential oils in the stages assayed. Essential oils showed strong larvicidal (LC₅₀, 24–36 µg mL⁻¹) and adulticidal (35–48 µg mL⁻¹) activities in mosquito species with an LT₅₀ of 4.5 h and 25–35 min, respectively. The evaluation of β-pinene produced a significant mortality rate (p < 0.01) in larvae whereas germacrene D was the most effective volatile (p < 0.01) against adults of both mosquito species. According to our results, β-pinene was the most effective volatile against the four insect species evaluated and its effect was comparable to that of the essential oil.

控制昆虫媒介的预防措施被认为是减少昆虫传播疾病发生率的最佳选择。本文中，我们报道了分布在墨西哥南部热带地区的药用植物Marina neglecta的叶精油的挥发性成分。为了调查香精油的化学变化，在2016-2019年的四个季节进行了挥发分筛查。同时，对它们在不同的生命周期中进行了测试，它们分别是麦加氏菌，巴氏支原体，埃及伊蚊和白纹伊蚊。。香精油主要由β-pine烯（> 30％），β-石竹烯（> 25％）和germ（D）（> 13％）组成。背腹部上的精油在三atom素上的应用，显示出有效的LC _50，适用于I至III级若虫（4 µg /只昆虫），IV至V级若虫（5–6 µg /只昆虫）和成年（7 –8 µg /昆虫）。I至III阶段的LT ₅₀在6至8小时之间，而IV至V阶段和成年人的LT ₅₀分别在12至16 h和22至26 h之间振荡。对若虫V进行的熏蒸实验表明，在处理72小时后，300 µg L ^-1空气产生100％的死亡率。在测试的挥发物中，β-pine烯和β-石竹烯的死亡率可比（p <0.01）比所分析的阶段中的精油要低。精油表现出较强的杀幼虫（LC ₅₀，24-36微克毫升^-1）和adulticidal（35-48微克毫升^-1）活动蚊种与LT ₅₀分别为4.5小时，25-35分钟，。β-of烯的评估 在幼虫中产生了显着的死亡率（p <0.01），而 对两种蚊虫的成虫而言，胚芽烯D是最有效的挥发物（p <0.01）。根据我们的研究结果，β-pine烯是对所评估的四种昆虫最有效的挥发物，其作用与精油相当。