The potential utilization of corn bran acid hydrolysate (CBAH) was evaluated as an inexpensive feedstock for the production of a rich carbohydrate and protein medium for lasiodiplodan (LAS) production using the filamentous fungus Lasiodiplodia theobromae CCT 3966. Experiments were performed according to a 2² CCRD experimental design aiming to evaluate the influence of agitation speed (rpm) and temperature (°C) over the production of total cell biomass (TCB) and LAS concentration released to the medium (LAS-M), adhered to biomass (LAS-C), and total (LAS-T). Under the selected conditions (temperature of 28°C and agitation of 200 rpm), 8.73 g·L⁻¹ of LAS-T and 4.47 g·L⁻¹ of TCB were obtained. Recovery of LAS-C with hot water was shown as an alternative to increase the production concentration, although it might require further purification steps. CBAH potential for substitution of synthetic media was demonstrated, indicating that it is an adequate raw material containing all necessary nutrients for LAS production.

Key points

• Corn bran acid hydrolysate is presented as a suitable substrate for β-glucan production.

• Lasiodiplodia theobromae CCT 3966 have the potential for the industrial β-glucan production.

• Simple recovering of biomass-adhered lasiodiplodan by hot water extraction.

Graphical abstract

中文翻译：

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Latheodiplodia theobromae CCT 3966由玉米糠酸水解产物生产β-葡聚糖胞外多糖lasiodiplodan

使用丝状真菌Lasiodiplodia theobromae CCT 3966 ，评估了玉米麸皮酸水解物（CBAH）的潜在利用作为廉价原料，以生产用于Lasiodiplodan（LAS）的丰富碳水化合物和蛋白质培养基。按照2 ²进行实验CCRD实验设计旨在评估搅拌速度（rpm）和温度（°C）对总细胞生物量（TCB）的产生以及释放到培养基（LAS-M），粘附到生物质（LAS-C）的LAS浓度的影响）和总计（LAS-T）。在选定的条件下（温度为28°C，搅拌速度为200 rpm），LAS-T的含量为8.73 g·L ^-1和4.47 g·L ^-1获得了TCB。尽管可能需要进一步的纯化步骤，但显示出用热水回收LAS-C作为增加生产浓度的替代方法。证明了CBAH替代合成培养基的潜力，表明它是包含LAS生产所需的所有营养素的充足原料。

关键点

•玉米麸皮酸水解物被认为是生产β-葡聚糖的合适底物。

•Lasiodiplodia theobromae CCT 3966具有工业化β-葡聚糖生产的潜力。

•通过热水提取简单地回收附着有生物质的拉索地龙。

图形概要