Burned human skin, which is routinely excised and discarded, contains viable mesenchymal stromal/stem cells (burn-derived mesenchymal stromal/stem cells; BD-MSCs). These cells show promising potential to enable and aid wound regeneration. However, little is known about their cell characteristics and biological function. This study had two aims: first, to assess critical and cellular characteristics of BD-MSCs and, second, to compare those results with multipotent well-characterized MSCs from Wharton’s jelly of human umbilical cords (umbilical cord mesenchymal stromal/stem cells, UC-MSCs). BD- and UC-MSCs were compared using immunophenotyping, multi-lineage differentiation, seahorse analysis for glycolytic and mitochondrial function, immune surface markers, and cell secretion profile assays. When compared to UC-MSCs, BD-MSCs demonstrated a lower mesenchymal differentiation capacity and altered inflammatory cytokine secretomes at baseline and after stimulation with lipopolysaccharides. No significant differences were found in population doubling time, colony formation, cell proliferation cell cycle, production of reactive oxygen species, glycolytic and mitochondrial function, and in the expression of major histocompatibility complex I and II and toll-like receptor (TLR). This study reveals valuable insights about MSCs obtained from burned skin and show comparable cellular characteristics with UC-MSCs, highlighting their potentials in cell therapy and skin regeneration.

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皮肤灼伤产生的干细胞的生物学特性—脐带干细胞的比较研究

经常切除并丢弃的烧伤的人类皮肤含有活的间充质基质/干细胞（烧伤衍生的间充质基质/干细胞； BD-MSC）。这些细胞显示出实现和帮助伤口再生的有希望的潜力。但是，关于它们的细胞特性和生物学功能知之甚少。这项研究有两个目标：首先，评估BD-MSC的关键和细胞特征；其次，将这些结果与沃顿顿人脐带果冻（脐带间充质基质/干细胞，UC- MSC）。使用免疫表型，多谱系分化，用于糖酵解和线粒体功能的海马分析，免疫表面标记和细胞分泌特征分析对BD-和UC-MSC进行了比较。与UC-MSC相比，BD-MSC在基线和脂多糖刺激后表现出较低的间充质分化能力并改变了炎性细胞因子分泌组。在种群倍增时间，菌落形成，细胞增殖细胞周期，活性氧的产生，糖酵解和线粒体功能以及主要组织相容性复合物I和II和toll样受体（TLR）的表达方面，均未发现显着差异。这项研究揭示了从烧伤皮肤获得的MSC的宝贵见解，并显示了与UC-MSC相当的细胞特性，突出了它们在细胞治疗和皮肤再生中的潜力。在群体倍增时间，菌落形成，细胞增殖细胞周期，活性氧的产生，糖酵解和线粒体功能以及主要组织相容性复合物I和II和toll样受体（TLR）的表达中均未发现显着差异。这项研究揭示了从烧伤皮肤获得的MSC的宝贵见解，并显示了与UC-MSC相当的细胞特性，突出了它们在细胞治疗和皮肤再生中的潜力。在种群倍增时间，菌落形成，细胞增殖细胞周期，活性氧的产生，糖酵解和线粒体功能以及主要组织相容性复合物I和II和toll样受体（TLR）的表达方面，均未发现显着差异。这项研究揭示了从烧伤皮肤获得的MSC的宝贵见解，并显示了与UC-MSC相当的细胞特性，突出了它们在细胞治疗和皮肤再生中的潜力。