当前位置: X-MOL 学术Analyst › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A label-free immunosensor for the detection of a new lung cancer biomarker, GM2 activator protein, using a phosphomolybdic acid/polyethyleneimine coated gold nanoparticle composite
Analyst ( IF 4.2 ) Pub Date : 2021-1-27 , DOI: 10.1039/d0an02149k
Kulrisa Kuntamung 1 , Padchanee Sangthong , Jaroon Jakmunee , Kontad Ounnunkad
Affiliation  

In this work, we report, for the first time, the construction of a label-free electrochemical immunosensor for highly sensitive detection of a new lung cancer biomarker, GM2 activator protein (GM2AP). A polyethyleneimine-coated gold nanoparticle (PEI-AuNP) and phosphomolybdic acid (PMA) modified electrode is developed as a novel redox platform for GM2AP detection. A PEI-AuNP film-modified screen-printed carbon electrode, as a signal amplifier support, was successfully fabricated for the adsorption of PMA redox molecules and is used for signal amplification. Under the optimized conditions, GM2AP detection is based on a decrease in the current response of PMA redox probes proportionally relative to an amount of the immunocomplex. Our sensor exhibits two linear ranges of 0.005–25 and 25–400 ng mL−1 with a limit of detection (LOD) of 0.51 pg mL−1. The immunosensor is successfully applied for the determination of GM2AP in both human urine and serum samples. The proposed sensor offers the advantages of simple fabrication, low cost, rapid analysis, satisfactory stability, high selectivity and sensitivity, and good reproducibility. The LOD of the biosensor is approximately 2863 and 1804 fold lower than the clinically relevant levels in human urine and serum, respectively. Our strategy can be used as an alternative non-invasive clinical analysis method for lung cancer screening.

中文翻译:

一种无标记免疫传感器,用于检测新的肺癌生物标志物 GM2 激活蛋白,使用磷钼酸/聚乙烯亚胺包覆的金纳米颗粒复合材料

在这项工作中,我们首次报道了一种无标记电化学免疫传感器的构建,用于高灵敏度检测新的肺癌生物标志物 GM2 激活蛋白 (GM2AP)。开发了一种聚乙烯亚胺包覆的金纳米颗粒 (PEI-AuNP) 和磷钼酸 (PMA) 修饰电极,作为一种用于 GM2AP 检测的新型氧化还原平台。成功制备了PEI-AuNP薄膜修饰的丝网印刷碳电极作为信号放大器载体,用于吸附PMA氧化还原分子并用于信号放大。在优化的条件下,GM2AP 检测基于 PMA 氧化还原探针的电流响应与免疫复合物的量成比例地降低。我们的传感器具有 0.005–25 和 25–400 ng mL -1的两个线性范围检测限 (LOD) 为 0.51 pg mL -1。该免疫传感器已成功应用于人尿液和血清样品中 GM2AP 的测定。所提出的传感器具有制造简单、成本低、分析快速、稳定性好、选择性和灵敏度高、重现性好等优点。生物传感器的 LOD 分别比人尿液和血清中的临床相关水平低约 2863 和 1804 倍。我们的策略可以用作肺癌筛查的替代非侵入性临床分析方法。
更新日期:2021-02-17
down
wechat
bug