Recently, lateral flow assay (LFA) for nucleic acid detection has drawn increasing attention in the point-of-care testing fields. Due to its rapidity, easy implementation, and low equipment requirement, it is well suited for use in rapid diagnosis, food authentication, and environmental monitoring under source-limited conditions. This review will discuss two main research directions of lateral flow nucleic acid tests. The first one is the incorporation of isothermal amplification methods with LFA, which ensures an ultra-high testing sensitivity under non-laboratory conditions. The two most commonly used methodologies will be discussed, namely Loop-mediated Isothermal Amplification (LAMP) and Recombinase Polymerase Amplification (RPA), and some novel methods with special properties will also be introduced. The second research direction is the development of novel labeling materials. It endeavors to increase the sensitivity and quantifiability of LFA testing, where signals can be read and analyzed by portable devices. These methods are compared in terms of limits of detection, detection times, and quantifiabilities. It is anticipated that future research on lateral flow nucleic acid tests will focus on the integration of the whole testing process into a microfluidic system and the combination with molecular diagnostic tools such as clustered regularly interspaced short palindromic repeats to facilitate a rapid and accurate test.

中文翻译：

---

用于核酸检测的侧流免疫测定法的快速发展

最近，用于核酸检测的侧向流动分析（LFA）在即时检测领域受到了越来越多的关注。由于其速度快，易于实施且设备要求低，因此非常适合在源受限的条件下用于快速诊断，食品认证和环境监测。本文将讨论侧向流动核酸测试的两个主要研究方向。第一个是将等温扩增方法与LFA结合使用，可确保在非实验室条件下的超高测试灵敏度。将讨论两种最常用的方法，即环介导的等温扩增（LAMP）和重组酶聚合酶扩增（RPA），还将介绍一些具有特殊性质的新颖方法。第二个研究方向是新型标签材料的开发。它致力于提高LFA测试的灵敏度和可量化性，在LFA测试中，便携式设备可以读取和分析信号。根据检测限，检测时间和可定量性对这些方法进行了比较。预计未来对侧向流核酸测试的研究将集中在将整个测试过程集成到微流体系统中，并与分子诊断工具（如成簇的规则间隔的短回文重复序列）相结合，以促进快速准确的测试。根据检测限，检测时间和可定量性对这些方法进行了比较。预计未来对侧向流核酸测试的研究将集中在将整个测试过程集成到微流体系统中，并与分子诊断工具（如成簇的规则间隔的短回文重复序列）相结合，以促进快速准确的测试。根据检测限，检测时间和可定量性对这些方法进行了比较。预计未来对侧向流核酸测试的研究将集中在将整个测试过程集成到微流体系统中，并与分子诊断工具（如成簇的规则间隔的短回文重复序列）相结合，以促进快速准确的测试。