MicroRNAs (miRNAs) play important roles in biological processes. Designing a sensitive, selective, and rapid method of miRNA detection is crucial for biological research. Here, with a reciprocal signal amplification (RSA) probe, this work established a novel surface-enhanced Raman scattering (SERS)-microfluidic approach for the quantitative analysis of miRNA. First, via a DNAzyme self-assemble cycle reaction, two types of SERS signals produce amplified reciprocal changes. The sum of the absolute signal value is first adopted for the quantitative analysis of miRNA, which results in an enhanced response and a reduced blank value. Furthermore, the assay is integrated in an electric drive microfluidic mixing reactor that enables physical mixing and enriching of the reactants for more rapid and enhanced detection sensitivity. The protocol owns the merits of the SERS technology, amplified reciprocal signals, and a microfluidic chip, with a detection limit of 2.92 fM for miR-141 in 40 min. In addition, successful determination of miRNA in a variety of cells proved the practicability of the assay. Compared with the reported strategies for miRNA analysis, this work avoids a complex and time-consuming procedure and enhances the sensitivity and specificity. The method opens a promising way for biomolecular chip detection and research.

中文翻译：

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SERS-微流控方法定量使用DNAzyme介导的相互信号放大的miRNA。

微小RNA（miRNA）在生物学过程中起重要作用。设计灵敏，选择性和快速的miRNA检测方法对于生物学研究至关重要。在这里，用双向信号放大（RSA）探针，这项工作建立了一种新颖的表面增强拉曼散射（SERS）-微流体方法，用于miRNA的定量分析。首先，通过DNAzyme自组装循环反应，两种类型的SERS信号会产生放大的倒数变化。绝对信号值的总和首先用于miRNA的定量分析，这导致增强的响应和降低的空白值。此外，该测定法集成在电驱动微流体混合反应器中，该反应器能够进行物理混合和富集反应物，从而实现更快，更高的检测灵敏度。该协议具有SERS技术的优点，可逆信号放大和微流控芯片，miR-141在40分钟内的检测限为2.92 fM。此外，成功测定多种细胞中的miRNA证明了该测定方法的实用性。与报道的miRNA分析策略相比，这项工作避免了复杂且耗时的过程，并提高了灵敏度和特异性。该方法为生物分子芯片的检测和研究开辟了一条有希望的途径。这项工作避免了复杂且耗时的过程，并提高了灵敏度和特异性。该方法为生物分子芯片的检测和研究开辟了有希望的途径。这项工作避免了复杂且耗时的过程，并提高了灵敏度和特异性。该方法为生物分子芯片的检测和研究开辟了有希望的途径。