ABSTRACT

1. The aim of the present study was to test the ability of an in-feed modified clinoptilolite zeolite-based mycotoxin binding substance (Minazel® Plus, Patent Co, Misicevo, Serbia; MP) to prevent gastrointestinal absorption of aflatoxin B₁ (AFB1) and ochratoxin A (OTA) and its effects on health status and performance parameters of broilers.

2. A total of 375, 1 d old male broiler chickens (Cobb 500) were used for a total trial period of 42 d (from hatch to 42 d of age). Animals were randomly allocated to five treatment groups (T1-T5), in 25 pens (15 male broilers per pen, five pens per treatment). T1 was the control maize-based diet without the addition of mycotoxins, or the test product. T2 and T3 groups received contaminated maize in the diet containing 0.02 mg AFB1/kg feed and 0.1 mg OTA/kg feed, whereas T4 and T5 groups received 0.05 mg AFB1/kg feed and 0.5 mg OTA/kg feed. The MP was added to T3 (1 g/kg feed), and T5 (2 g/kg feed) groups.

3. Results showed that exposure to AFB1 and OTA at low or moderate levels, as used in this study, did not markedly affect growth performance, blood profile or organ weights. Improvements in feed conversion ratio (FCR) were observed in birds receiving MP, whereby FCR of T3 group was improved in comparison with T2 group, although there was no significant difference between T5 and T4 groups. However, average body weight gain (ABWG) was improved in the T5 group compared to T4, but not in the T3 versus T2 group comparison.

4. For serum biochemical parameters, glutamate-dehydrogenase (GLDH) was significantly improved in T5 birds in comparison with T4. The addition of MP significantly decreased residue levels of AFB1 in liver and OTA in the spleen of the treated groups.

5. The improvements in productive performance and reduction of mycotoxin residue levels in tissues demonstrated a beneficial effect of MP in cases of concurrent AFB1 and OTA ingestion by broilers.

摘要

1. 本研究的目的是测试饲料中改性斜发沸石基霉菌毒素结合物质（Minazel® Plus，Patent Co，Misicevo，塞尔维亚；MP）防止黄曲霉毒素 B ₁（AFB1 ）胃肠道吸收的能力) 和赭曲霉毒素 A (OTA) 及其对肉鸡健康状况和性能参数的影响。

2. 总共使用了 375 只 1 日龄雄性肉鸡（Cobb 500），总试验期为 42 日（从孵化到 42 日龄）。动物被随机分配到五个处理组（T1-T5），在 25 个围栏中（每个围栏 15 只雄性肉鸡，每个处理五个围栏）。T1 是未添加霉菌毒素的对照玉米饮食或测试产品。T2 和 T3 组在含有 0.02 mg AFB1/kg 饲料和 0.1 mg OTA/kg 饲料的饲料中接受污染玉米，而 T4 和 T5 组接受 0.05 mg AFB1/kg 饲料和 0.5 mg OTA/kg 饲料。将 MP 添加到 T3（1 g/kg 饲料）和 T5（2 g/kg 饲料）组中。

3. 结果表明，本研究中使用的低或中等水平的 AFB1 和 OTA 暴露不会显着影响生长性能、血液特征或器官重量。在接受 MP 的鸡只中观察到饲料转化率（FCR）的改善，其中 T3 组的 FCR 与 T2 组相比有所提高，尽管 T5 和 T4 组之间没有显着差异。然而，与 T4 相比，T5 组的平均体重增加 (ABWG) 有所改善，但在 T3与T2 组的比较中没有。

4. 血清生化指标，谷氨酸脱氢酶（GLDH）在T5鸡只比T4鸡有显着改善。MP的添加显着降低了治疗组肝脏中AFB1和脾脏中OTA的残留水平。

5. 在肉鸡同时摄入 AFB1 和 OTA 的情况下，生产性能的改善和组织中霉菌毒素残留水平的降低证明了 MP 的有益作用。