ABSTRACT

Fatty acid desaturase 1 (FADS1) polymorphisms alter fatty acid content in subcutaneous adipose tissue (SAT); however, existing evidence is limited and conflicting regarding the association between FADS1 variants and SAT inflammatory status. To advance this area, we conducted an exploratory study to investigate whether the common rs174537 polymorphism in FADS1 was associated with immune cell profiles in abdominal and femoral SAT in individuals with obesity. FADS1 gene expression and immune cell profiles in SAT depots were assessed by qPCR and flow cytometry, respectively. Although FADS1 gene expression was associated with genotype, no associations were observed with immune cell profiles in either depot. Our study provides additional evidence that rs174537 in FADS1 has minimal impact on inflammatory status in obese SAT.

摘要

脂肪酸去饱和酶 1 ( FADS1 ) 多态性改变皮下脂肪组织 (SAT) 中的脂肪酸含量；然而，关于FADS1变异与 SAT 炎症状态之间的关联，现有证据有限且相互矛盾。为了推动这方面，我们进行了探索性研究，以调查是否在公共rs174537多态性FADS1用在与肥胖个体中腹和股SAT免疫细胞配置文件关联。分别通过 qPCR 和流式细胞术评估 SAT 库中的FADS1基因表达和免疫细胞谱。虽然FADS1基因表达与基因型相关，在任一仓库中均未观察到与免疫细胞谱的关联。我们的研究提供了额外的证据，即 FADS1 中的rs174537对肥胖 SAT 的炎症状态影响最小。