The AT-hook motif nuclear-localized (AHL) family is a plant transcription factor family, which plays an important role in growth and development and stress responses. We identified and analyzed 37 AHL genes in poplar (Populus trichocarpa). Phylogenetic analysis classified the PtrAHL members into three subfamilies based on their conserved domain. All PtrAHL paralogous pairs evolved under purifying selection. The promoter analysis revealed the presence of stress-related and phytohormone-related cis-elements of the PtrAHL genes. Our analysis of the tissue-specific expression pattern of PtrAHL genes indicated their significance in tissue and organ development. Network-based prediction suggested that PtrAHL genes may interact with histone deacetylases (HDAC) and participate in the development of organs, such as roots. Drought negatively impacts plant growth and development. ABA is produced under osmotic stress condition, and it takes an important part in the stress response and tolerance of plants. Real-time quantitative PCR (qRT-PCR) showed that PtrAHL genes were induced by drought stress and ABA treatment. These insights into the expression of PtrAHL genes under stress provide a basis for PtrAHL gene functional analysis. Our study will help develop new breeding strategies to improve drought tolerance in poplar.

中文翻译：

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毛果杨干旱胁迫和ABA处理下AHL基因家族及其表达的综合分析。

AT钩基序核定位（AHL）家族是一种植物转录因子家族，在生长发育和胁迫响应中起着重要作用。我们鉴定并分析了杨（Populus trichocarpa）中的37个AHL基因。系统发育分析根据其保守域将PtrAHL成员分为三个亚家族。所有PtrAHL同源对在纯化选择下进化。启动子分析揭示了PtrAHL基因的应激相关和植物激素相关顺式元件的存在。我们对PtrAHL基因的组织特异性表达模式的分析表明，它们在组织和器官发育中具有重要意义。基于网络的预测表明，PtrAHL基因可能与组蛋白脱乙酰基酶（HDAC）相互作用，并参与器官如根的发育。干旱对植物的生长和发育产生负面影响。ABA是在渗透胁迫条件下产生的，它在植物的胁迫反应和耐受性中起着重要的作用。实时定量PCR（qRT-PCR）表明，PtrAHL基因是由干旱胁迫和ABA处理诱导的。这些对压力下PtrAHL基因表达的见解为PtrAHL基因功能分析提供了基础。我们的研究将有助于开发新的育种策略，以提高杨树的耐旱性。这些对压力下PtrAHL基因表达的见解为PtrAHL基因功能分析提供了基础。我们的研究将有助于开发新的育种策略，以提高杨树的耐旱性。这些对压力下PtrAHL基因表达的见解为PtrAHL基因功能分析提供了基础。我们的研究将有助于开发新的育种策略，以提高杨树的耐旱性。