The utilisation of interstocks or rootstocks to reduce tree size has long been used in orchards. In the present study, we characterised the dwarfing effect of “OHF51” (Pyrus communis “Old Home” × “Farmingdale”) when used as interstock compared with callery pear (Pyrus calleryana Decne.). Shorter trees, smaller canopy, smaller trunk diameter and lower height of the first branch were found in trees with “OHF51” as the interstock as compared to callery pear. Metabolome analysis showed that the interstock altered the metabolite profiles of both the scion and the rootstock. The number of identified differential metabolites in scions, interstocks and roots were 8, 39 and 12, respectively, with “OHF51” interstock as compared to the same positions with callery as the interstock. Flavonoids and phenolic acids/derivatives were most altered in concentration. The concentrations of flavonoids procyanidin B2 and procyanidin B3 were remarkably higher in both the scion shoots and the interstock because of “OHF51,” while the concentration of two monosucroses, D-sorbitol and D-mannitol, were drastically reduced in roots with “OHF51” interstock present. The reallocation of carbohydrates from shoot to root could be influenced by the interstock, which participates in the regulation of scion growth in the spring season. Flavonoids and phenolic acids/derivatives are understood to be key compounds involved in the reduction of scion growth.

中文翻译：

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代谢组分析揭示了与interstock 对梨接穗的矮化作用相关的重要化合物

利用中间砧木或砧木来减小树木大小长期以来一直用于果园。在本研究中，我们描述了“OHF51”（Pyrus community “ Old Home” × “Farmingdale”）与callery pear ( Pyrus calleryana拒绝。）。与苦瓜梨相比，在以“OHF51”作为中间砧木的树木中发现了更短的树木、更小的树冠、更小的树干直径和更低的第一枝高度。代谢组分析表明，中间砧木改变了接穗和砧木的代谢物谱。在接穗、中间砧木和根中鉴定的差异代谢物的数量分别为 8、39 和 12 个，“OHF51”中间砧木与相同位置的砧木和中间砧木相比。类黄酮和酚酸/衍生物的浓度变化最大。由于“OHF51”的存在，接穗枝条和中间砧木中的类黄酮原花青素 B2 和原花青素 B3 的浓度显着更高，而两种单蔗糖 D-山梨糖醇和 D-甘露糖醇的浓度，在存在“OHF51”中间砧木的情况下，根部显着减少。碳水化合物从地上部到根的重新分配可能受到中间砧木的影响，中间砧木参与了春季接穗生长的调控。类黄酮和酚酸/衍生物被认为是参与减少接穗生长的关键化合物。