Defatted black soldier fly (Hermetia illucens) larvae meal (DBSFLM) has been shown an important alternative protein ingredient for fish, but little information is available about the effect of DBSFLM on growth and health of Litopenaeus vannamei. A 56-day feeding trial was conducted to assess the effects of partial replacement of fish meal with DBSFLM on growth performance, body composition, nutrition retention, blood metabolites, antioxidant and immune response, digestive enzyme activities and hepatic morphology of L. vannamei. Six isonitrogenous and isoenergy diets were formulated by replacing 0% (G0), 15% (G15), 30% (G30), 45% (G45), 60% (G60) and 80% (G80) of fish meal with DBSFLM. Each diet was randomly assigned to quadruplicate groups of 40 shrimp per tank. Shrimp were fed three times daily to apparent satiation. Blood samples were collected at the terminal trial to analyse antioxidant and non-specific immune enzyme activities. Hepatopancreas and intestine samples were collected to determine digestive enzyme activities and hepatic morphology. Shrimp fed G80 had lower (p < .05) final body weight, weight gain and specific growth rate, and higher (p < .05) feed conversion ratio than other groups. Crude protein (CP) in the whole body and muscle, coupled with CP and lipid retention of shrimp were decreased (p < .05) as dietary DBSFLM increasing. Shrimp fed G80 had lower (p < .05) serum total cholesterol but higher (p < .05) total antioxidant capacity than other diets, and serum malondialdehyde was lower (p < .05) in shrimp fed G45-G80 than those fed G0-G30. Hepatic and intestinal digestive enzyme activities were not altered by treatments (p > .05). Hepatocytes were shown to have different degrees of vacuolar degeneration, hepatic corpuscle atrophy and starlike lumen lose as observed in G60 and G80. The results demonstrated that up to 60% of fish meal could be replaced by DBSFLM without any adverse effects on growth performance, antioxidant and immune enzyme activities, and digestive enzyme activities of L. vannamei. However, hepatocyte damage was observed when the replacement levels exceeded 60%.

中文翻译：

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南美白对虾脱脂 Hermetia illucens 幼虫粉的评价：对生长性能、营养保留、抗氧化和免疫反应、消化酶活性和肝脏形态的影响

脱脂黑水虻( Hermetia illucens ) 幼虫粉 (DBSFLM) 已被证明是鱼类的重要替代蛋白质成分，但关于 DBSFLM 对南美白对虾生长和健康的影响的信息很少。A 56天喂养试验以评估与DBSFLM部分替代鱼粉的生长性能，身体组成，营养滞留，血液代谢物，抗氧化剂和免疫反应，消化酶活性和肝形态学的影响大号。 南美白对虾. 通过用 DBSFLM 代替 0% (G0)、15% (G15)、30% (G30)、45% (G45)、60% (G60) 和 80% (G80) 的鱼粉，配制了六种等氮和等能日粮。每种饮食被随机分配到每缸 40 只虾的四组。每天喂虾 3 次以达到明显的饱腹感。在最终试验中收集血液样本以分析抗氧化和非特异性免疫酶活性。收集肝胰腺和肠道样本以确定消化酶活性和肝脏形态。G80 喂养的虾的 最终体重、体重增加和比生长率较低 ( p < .05)，而较高 ( p < .05) 饲料转化率高于其他组。 随着日粮 DBSFLM 的增加，整个身体和肌肉中的粗蛋白 (CP) 以及虾的 CP 和脂质保留降低 ( p < .05)。 与其他饮食相比，G80 喂养的虾的 血清总胆固醇较低 ( p < .05) 但总抗氧化能力较高 ( p < .05)，并且 G45-G80 喂养的虾的血清丙二醛低于G0 喂养的虾( p < .05) -G30。肝和肠消化酶活性未因治疗而改变（p > .05）。肝细胞表现出不同程度的空泡变性、肝小体萎缩和星状管腔丢失，如在 G60 和 G80 中观察到的。结果表明，高达 60% 的鱼粉可以被 DBSFLM 替代，而不会对L 的生长性能、抗氧化和免疫酶活性以及消化酶活性产生任何不利影响。 南美白对虾。然而，当替代水平超过 60% 时，观察到肝细胞损伤。