Dendrobium hybrids have been cultivated as commercially important ornamental plants in the floriculture industry. However, the potential of Dendrobium hybrids as a source of valuable secondary metabolites has not been fully exploited. In this study, the effect of various elicitors on the proliferation of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue and the corresponding production of dendrobine and anthocyanin was investigated. Yeast extract (YE) and glutamine (both at 200 mg L⁻¹) increased dendrobine content in PLBs by 2.7- and 6.5-fold (54.874 ± 2.553 and 894.481 ± 26.185 ng mg⁻¹dry weight (DW)) compared to controls (20.051 ± 0.182 and 136.956 ± 7.397 ng mg⁻¹ DW). Glutamine (200 mg L⁻¹) also significantly enhanced PLB biomass (0.190 ± 0.009 g) with respect to the control (0.144 ± 0.003 g). Chitosan and salicylic acid (SA) were not effective in enhancing the dendrobine and anthocyanin contents in PLBs. A combination of 4 mg L⁻¹ NAA and 100 mg L⁻¹ arginine increased dendrobine content by 8-fold (123.793 ± 5.446 ng mg⁻¹ DW) compared to the control (15.554 ± 0.406 ng mg⁻¹ DW). Anthocyanin content was increased by 1.7-fold (0.548 ± 0.019 Color Value (CV) g⁻¹ DW) when PLBs treated with 4 mg L⁻¹ TDZ were exposed to 1.5 h of UV irradiation as compared to those without UV treatment (0.331 ± 0.019 CV g⁻¹ DW). Despite the low dendrobine content, PLBs as a source of dendrobine remains appealing due to its short culture duration (6 wk) compared to mother plants (2 yr).

石end杂种已被培育为花卉栽培业中商业上重要的观赏植物。但是，石end杂种作为有价值的次级代谢产物来源的潜力尚未得到充分利用。在这项研究中，在球茎状体（小巴）的增殖的各种激发子的效果石斛萨宾蓝和dendrobine和花青素的相应的生产进行了研究。与对照相比，酵母提取物（YE）和谷氨酰胺（均为200 mg L ^-1）使小灵通中的石d碱含量增加了2.7倍和6.5倍（54.874±2.553和894.481±26.185 ng mg ^-1干重（DW））。 20.051±0.182和136.956±7.397 ng mg ^-1 DW）。谷氨酰胺（200毫克L^-1）相对于对照（0.144±0.003 g）也显着增强了PLB生物量（0.190±0.009 g）。壳聚糖和水杨酸（SA）不能有效提高PLB中的石den碱和花色苷含量。为4mg L A组合^-1 NAA和100毫克的L ^-1精氨酸通过增加dendrobine内容8倍（123.793±5.446纳克毫克^-1 DW）与对照相比（15.554±0.406纳克毫克^-1 DW）。与未进行紫外线处理的PLB相比，使用4 mg L ^-1 TDZ处理的PLB暴露于紫外线照射1.5 h时，花色苷含量增加了1.7倍（0.548±0.019色值（CV）g ^-1 DW）0.019 CV克^-1DW）。尽管石rob碱含量低，但由于PLB的培养持续时间短（6周），而与母体植物（2年）相比，作为石d碱的来源仍然具有吸引力。