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Aptamer-immobilized Gold Nanoparticles Enable Facile and On-site Detection of Staphylococcus aureus
Biotechnology and Bioprocess Engineering ( IF 3.2 ) Pub Date : 2021-02-17 , DOI: 10.1007/s12257-020-0161-z
Seong Ha Lim , Yeong Chae Ryu , Byeong Hee Hwang

Staphylococcus aureus causes numerous illnesses and national loss in many countries. Therefore, to prevent food poisoning outbreaks, a rapid and straightforward on-site detection method is required without special skills or equipment. Here, we used aptamer-immobilized gold nanoparticles (aiGNPs) for the on-site colorimetric S. aureus detection. For the rapid and facile detection of S. aureus, the SA61 aptamer hybridized with thiol-modified adaptors conjugated onto the GNP surface of approximately 13 nm diameter. The synthesis of GNPs was confirmed using a transmission electron microscopy and energy dispersive spectroscopy. Images using a scanning electron microscope showed attachment of aiGNPs to S. aureus. After 30-min incubation and 20-min centrifugation, the red pellet was specifically observed with the sensitivity of OD600 0.050 equivalent to 7.5 × 106 cells/mL. Besides, aiGNPs could accurately detect S. aureus with a detection limit of OD600 0.10 equivalent to 1.5 × 107 cells/mL even in the mixed sample with Escherichia coli. Quantitative analysis was performed between relative area and cell concentration. Because this method enables fast and specific S. aureus detection without special skills or expensive equipment, aiGNPs could enable a rapid and effective on-site detection of S. aureus to prevent pathogenic outbreaks.



中文翻译:

适体固定的金纳米粒子可实现金黄色葡萄球菌的便捷和现场检测

金黄色葡萄球菌在许多国家引起许多疾病和国民损失。因此,为防止食物中毒暴发,需要一种快速而直接的现场检测方法,而无需特殊技能或设备。在这里,我们使用适体固定金纳米颗粒(aiGNPs)进行现场比色金黄色葡萄球菌检测。为了快速,简便地检测金黄色葡萄球菌,将SA61适体与结合到直径约13 nm的GNP表面的巯基修饰的衔接子杂交。使用透射电子显微镜和能量分散光谱法证实了GNP的合成。使用扫描电子显微镜的图像显示aiGNP附着在金黄色葡萄球菌上。孵育30分钟和离心20分钟后,以OD 600 0.050的等效灵敏度7.5×10 6细胞/ mL观察到红色沉淀。此外,即使在与大肠杆菌的混合样品中,aiGNPs仍可以准确检测出金黄色葡萄球菌,其检出限为OD 600 0.10,相当于1.5×10 7细胞/ mL 。在相对面积和细胞浓度之间进行定量分析。因为此方法无需特殊技能或昂贵的设备即可进行快速,特异性的金黄色葡萄球菌检测,所以aiNPNP可以实现对金黄色葡萄球菌的快速,有效现场检测,以防止病原体爆发。

更新日期:2021-02-17
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