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Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies
BioChip Journal ( IF 4.3 ) Pub Date : 2021-02-17 , DOI: 10.1007/s13206-021-00011-6
Ji-Hong Bong 1 , Tae-Hun Kim 1 , Jaeyong Jung 1 , Soo Jeong Lee 1 , Jeong Soo Sung 1 , Chang Kyu Lee 1 , Min-Jung Kang 2 , Hyun Ok Kim 3 , Jae-Chul Pyun 1
Affiliation  

Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.



中文翻译:

使用猪血清衍生的抗 SARS-CoV-2 抗体对 SARS-CoV-2 进行竞争性免疫测定

使用人 SARS-CoV-2 NP 固定磁珠从猪血清中分离出抗严重急性呼吸综合征冠状病毒 2(抗 SARS-CoV-2)核蛋白(NP)抗体。使用 SARS-CoV-2 NP 固定磁珠通过流式细胞仪测试分离的针对 SARS-CoV-2 NP 的抗体的结合特性。开发了一种竞争性免疫测定法,使用带有固定化抗 SARS-CoV-2 NP 抗体的磁珠检测培养液中的 SARS-CoV-2 NP 和 SARS-CoV-2。在对培养液中的 SARS-CoV、MERS-CoV 和 CoV 株 229E 进行竞争性免疫测定期间进行了选择性测试。

更新日期:2021-02-17
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