The family-level relationships within Plecoptera have been a focused area of research for a long time. Its higher classification remains unstable, and the phylogenetic relationships within Plecoptera should be re-examined. Here, we sequenced and analyzed two complete mitochondrial genomes (mitogenomes) of Paraleuctra cercia and Perlomyia isobeae of the family Leuctridae. We reconstructed the phylogeny of Plecoptera based on 13 protein-coding genes (PCGs) from published stoneflies. Our results showed that the Bayesian inference and maximum-likelihood tree had similar topological structures except for the positions of two families, Peltoperlidae and Scopuridae. The Plecoptera is divided into two clades, the suborder Antarctoperlaria and the suborder Arctoperlaria. The two suborders subsequently formed two groups, Eusthenioidea and Gripopterygoidea, and Euholognatha and Systellognatha, which is consistent with the results of morphological studies. In addition, the Leuctridae is the earliest branch within the superfamily Nemouroidea. But the monophyly of Perloidea and Pteronarcyoidea are still not well supported.

中文翻译：

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来自 Leuctridae (Plecoptera: Nemouroidea) 的两个完整线粒体基因组：对石蝇系统发育关系的影响

长期以来，Plecoptera 中的家庭级关系一直是研究的重点领域。其更高的分类仍然不稳定，应重新检查 Plecoptera 内部的系统发育关系。在这里，我们对 Leuctridae 的 Paraleuctra cercia 和 Perlomyia isobeae 的两个完整的线粒体基因组（有丝分裂基因组）进行了测序和分析。我们根据已发表的石蝇的 13 个蛋白质编码基因 (PCG) 重建了 Plecoptera 的系统发育。我们的结果表明，贝叶斯推理和最大似然树具有相似的拓扑结构，除了两个科的位置，Peltoperlidae 和 Scopuridae。翅翅目分为两个进化枝，南极鱼亚目和北极鱼亚目。这两个亚目随后形成了两个组，Eusthenioidea 和 Gripopterygoidea，和 Euholognatha 和 Systellognatha，这与形态学研究的结果是一致的。此外，Leuctridae 是 Nemouroidea 超科中最早的分支。但是 Perloidea 和 Pteronarcyoidea 的单系仍然没有得到很好的支持。