Abstract

The purpose of this study is to prepare stimuli-responsive chimeric liposomes (i.e. hybrid polymer-lipid liposomes) containing functional copolymers and conduct aqueous solution studies in order to determine their properties and potential as drug-delivery nanocarriers. Two random copolymers, composed of the hydrophilic, pH and thermo-responsive 2-(dimethyl amino) ethyl methacrylate (DMAEMA) monomer and the hydrophobic stearyl methacrylate (SMA) monomer, were synthesized via free-radical polymerization and molecularly characterized using SEC, FTIR, and 1H-NMR. The synthesis was followed by aqueous solution studies, utilising dynamic light scattering (DLS) in order to determine their stimuli responsive self-assembly properties. The preparation of chimeric liposomes was mediated by thin film deposition and hydration, followed by aqueous solution studies via DLS, ζ-potential and fluorescence spectroscopy. The drug-loading studies include curcumin loading via a thin film deposition and hydration technique, while aqueous solution properties of the drug-loaded chimeric liposomes were determined utilizing DLS, and UV–Vis spectroscopy.

摘要

本研究的目的是制备含有功能共聚物的刺激响应嵌合脂质体（即混合聚合物-脂质脂质体）并进行水溶液研究，以确定它们作为药物递送纳米载体的特性和潜力。通过自由基聚合合成了两种由亲水性、pH 值和热响应性 2-(二甲基氨基) 甲基丙烯酸乙酯 (DMAEMA) 单体和疏水性甲基丙烯酸硬脂酯 (SMA) 单体组成的无规共聚物，并使用 SEC、FTIR 进行分子表征, 和 1 H-NMR。合成之后是水溶液研究，利用动态光散射 (DLS) 以确定它们的刺激响应自组装特性。嵌合脂质体的制备是通过薄膜沉积和水合介导的，随后通过 DLS、ζ 电位和荧光光谱学进行水溶液研究。载药研究包括通过薄膜沉积和水合技术加载姜黄素，而载药嵌合脂质体的水溶液特性则使用 DLS 和紫外-可见光谱法确定。