Abstract

A novel cyanobacterial species from the genus Iningainema is described according to the International Code of Nomenclature for Algae, Fungi, and Plants. The new species, Iningainema sahyadrensis sp. nov., is recorded from the Western Ghats (Sahyadri mountain range), India. The description is based on morphological features and the phylogenetic analysis of partial 16S rDNA gene. Phylogenetic analysis places I. sahyadrensis in the Iningainema clade, within the family Scytonemataceae. The novel strain formed a strongly supported monophyletic lineage with strains of the type species of Iningainema, I. pulvinus. Laboratory-grown culture and the naturally occurring samples of I. sahyadrensis differed from the type species of the genus (I. pulvinus) by the size of vegetative cells, thickness of sheath, presence of vacuole-like structures in cells and habitat type. Comparison of predicted secondary structures of the partial 16S rRNA molecule of I. sahyadrensis and three clones of I. pulvinus showed a number of evolutionary events in Domain 1 (H8-H10, H17), Domain 2 (H21, H22) and Domain 3 (H33, H34). Amplification of a fragment of the ndaF gene involved in nodularin biosynthesis from I. sahyadrensis did not confirm that it has this genetic determinant, in contrast with I. pulvinus. The recognition and description of this novel species suggests that the cyanobacterial flora of mountain habitats of India has yet to be fully documented.

摘要

根据国际藻类、真菌和植物命名法典描述了一种来自Iningainema属的新蓝藻物种。新种Iningainema sahyadrensis sp。11 月，记录自印度西高止山脉（Sahyadri 山脉）。该描述基于部分 16S rDNA 基因的形态特征和系统发育分析。系统发育分析将I. sahyadrensis置于Scytonemataceae科的Iningainema进化枝中。该新菌株与Iningainema, I. pulvinus 的模式种的菌株形成了强烈支持的单系谱系。实验室培养的培养物和I. sahyadrensis的天然样本在营养细胞的大小、鞘的厚度、细胞中液泡状结构的存在和栖息地类型方面与该属的模式种（I. pulvinus）不同。的部分的16S rRNA分子的预测的二级结构的比较I. sahyadrensis和的三个克隆I.叶枕表明在结构域1（H8-H10，H17），域2（H21，H22）和域3（一些进化事件的H33、H34）。与I. pulvinus相比，对参与来自I. sahyadrensis的节球藻素生物合成的ndaF基因片段的扩增并未证实它具有这种遗传决定因素. 这种新物种的识别和描述表明，印度山区栖息地的蓝藻菌群尚未完全记录。