The effects of human handling (friendly versus aversive) and milking frequency (once versus twice daily) on productive and behavioral parameters of two Greek dairy sheep breeds (Karagouniko and Chios) were examined. The experiment started a week after lamb weaning (50 ± 5 days after lamb birth) and lasted for ten weeks. Milk yield, milk flow rate and number of kick responses during milking were weekly recorded. Each milking was alternatively performed by a friendly or an aversive milker. At the end of 4th and 9th week, an isolation - flight distance test was also applied. As shown, Karagouniko ewes had lower daily milk yield but higher milk flow rate than Chios ewes. Higher milk yield was also observed in ewes milked twice compared to those milked once per day. Aversive handling increased the number of kick responses during milking and decreased milk yield. At the same time, ewes exhibited a preference for a specific position in the milking parlor. During the isolation - flight distance test, Karagouniko ewes bleated less, but had longer flight distances compared to Chios ones. Multiparous had a shorter flight distance than the primiparous ewes. Finally, ewes milked once per day displayed more jumps and less bleats compared to those milked twice. It is suggested that specific training programs targeted at the handling improvement with the intention to reduce fear responses could improve productivity in dairy ewes.

考察了人类操作（友好与厌恶）和挤奶频率（一次与每日两次）对两种希腊奶牛品种（Karagouniko和Chios）的生产和行为参数的影响。实验开始于断奶后一周（羔羊出生后50±5天），持续了十周。每周记录挤奶过程中的牛奶产量，牛奶流速和反冲次数。每次挤奶也可以由友善或厌恶的挤奶者进行。在第4周和第9周结束时，还进行了隔离-飞行距离测试。如图所示，Karagouniko母羊的日产奶量比Chios母羊低，但牛奶流率更高。与每天挤奶一次的母羊相比，挤奶两次的母羊的产奶量也更高。厌恶性处理增加了挤奶过程中反冲的次数，降低了产奶量。同时，母羊对挤奶厅中的特定位置表现出偏爱。在隔离-飞行距离测试中，Karagouniko母羊的出血少，但与Chios相比，飞行距离更长。杂母比初母母羊的飞行距离短。最后，与两次挤奶的母羊相比，每天挤奶一次的母羊跳动更大且脱臭更少。建议针对培训的改进以减少恐惧反应为目标的特定培训计划可以提高母乳的生产率。但与希俄斯相比，飞行距离更长。杂母比初母母羊的飞行距离短。最后，与两次挤奶的母羊相比，每天挤奶一次的母羊跳动更大且脱臭更少。建议针对培训的改进以减少恐惧反应为目标的特定培训计划可以提高母乳的生产率。但与希俄斯相比，飞行距离更长。杂母比初母母羊的飞行距离短。最后，与两次挤奶的母羊相比，每天挤奶的母羊表现出更多的跳跃和更少的。建议针对培训的改进以减少恐惧反应为目标的特定培训计划可以提高母乳的生产率。