The objective of this experiment was to evaluate the acaricidal activity of Senecio cannabifolius essential oil, and two of its constituents, eucalyptol and camphor. Efficacy against females and larvae of Rhipicephalus (Boophilus) microplus was assessed by the adult immersion test (AIT) and the larval immersion test (LIT). The oil was analyzed by gas chromatography-flame ionization detection (GC-FID) and GC-mass spectrometry (MS) and in total 68 components were identified representing 99.2% of the essential oil. AIT revealed that the oil, eucalyptol, and camphor at the highest concentration presented efficacy of 68.9, 57.1, and 71.9%, respectively. LIT revealed that the essential oil and camphor achieved 100% mortality at concentration of 1.6% wt/vol, whereas eucalyptol showed moderate inhibitory activity. Biochemical assays indicated that the essential oil and camphor can reduce significantly overall detoxification enzyme activities in engorged females and larvae at high concentration (≥ 0.4% wt/vol), whereas the inhibitory effect of eucalyptol is weaker than that of the oil and camphor. Taken together our results indicated that the S. cannabifolius essential oil and its isolated constituent had potential for the development of a new and safe acaricide for the control of R. microplus ticks.

该实验的目的是评估千里光大麻油及其两种成分桉树油和樟脑的杀螨活性。微小头蛇耳（Rhipicephalus（Boophilus））雌虫和幼虫的功效通过成人浸没测试（AIT）和幼虫浸没测试（LIT）进行评估。通过气相色谱-火焰离子化检测（GC-FID）和GC-质谱（MS）分析该油，共鉴定出68种成分，占精油的99.2％。AIT透露，最高浓度的精油，桉树精和樟脑的功效分别为68.9％，57.1和71.9％。LIT显示，精油和樟脑在1.6％wt / vol的浓度下可达到100％的死亡率，而桉树油则表现出中等的抑制活性。生化分析表明，在高浓度（≥0.4％wt / vol）的情况下，精油和樟脑可以显着降低饱食的雌性和幼虫的总排毒酶活性，桉树油的抑制作用比油和樟脑的抑制作用弱。综合我们的结果，我们发现大麻链球菌精油及其分离的成分具有开发新型和安全的杀螨剂的潜力，可用于控制微小micro。