BACKGROUND:Black currants are berries that attract attention due to the beneficial effects they possess. They are proven to be good antioxidants, anticancerogenic, cardio- and neuroprotective agents. OBJECTIVE:The main objective of this research was to evaluate the differences in the flavonols composition, antioxidant and spasmolytic activity of juices obtained from four different black currant cultivars – Triton, Tenah, Ben Sarek, and Ometa, grown over a 4-year long period on the territory of Serbia. METHODS:The qualitative and quantitative determination of black currants juices flavonols was performed using the HPLC. The antioxidative activity was carried out in DPPH and β-carotene systems. The study examined the impact of the main black currants flavonols and black currants juices on the spontaneous, KCl, and acetylcholine-induced ileum smooth muscle contractions. RESULTS:The dominant flavonol in juices of the four different black currant cultivars was myricetin, with its highest content obtained from the 2015 Triton juice (820.1±34.7 μg g–1). Quercetin was the second in abundance and the lowest yields were observed in kaempferol. CONCLUSION:The investigated black currant cultivars were proven to be good sources of flavonols and potential antioxidants with the significant spasmolytic activity that could be successfully used in treating gastrointestinal disorders.

中文翻译：

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黑醋栗黄酮醇成分及其对解痉活性的影响

背景：黑加仑子是浆果，由于其具有的有益作用而引起人们的关注。它们被证明是良好的抗氧化剂，抗癌剂，心脏和神经保护剂。目的：本研究的主要目的是评估从四个不同的黑加仑品种（Triton，Tenah，Ben Sarek和Ometa）获得的，经过4年的长期种植的果汁中黄酮醇成分，抗氧化剂和解痉活性的差异。在塞尔维亚境内。方法：采用HPLC法对黑加仑汁中的黄酮醇进行定性和定量测定。抗氧化活性在DPPH和β-胡萝卜素体系中进行。该研究检查了主要黑加仑黄酮醇和黑加仑汁对自发性KCl的影响，和乙酰胆碱引起的回肠平滑肌收缩。结果：四个黑加仑品种果汁中的主要黄酮醇为杨梅素，含量最高的是2015年的Triton果汁（820.1±34.7μgg-1）。槲皮素含量第二高，而山奈酚的产量最低。结论：所研究的黑加仑品种被证明是黄酮醇和潜在的抗氧化剂的良好来源，具有显着的解痉活性，可成功用于治疗胃肠道疾病。