Abstract

The aim of this study is to determine the relationship between some growth and development characteristics in Anatolian Black cattle from birth to twelve months of age with the Pit-1, GHR and Myostatin genes. PCR-RFLP method was used to detect the polymorphism. Genotype and allele frequencies were found to be AA/AB/BB: 0.096/0.519/0.385 and A/B: 0.356/0.644; AA/AG/GG: 0.346/0.385/0.269 and A/G: 0.538/0.462 in the Pit-1 and GHR genes respectively. Myostatin gene was found to be also monomorphic in all genotypes. Although the chi-square χ² test in the Pit-1 gene showed an agreement to Hardy–Weinberg equilibrium (p > 0.05), in the GHR gene did not showed an agreement (p < 0.05). The results of the statistical analysis indicated an association between Pit-1 and GHR genes polymorphism and growth traits at different stage ages in Anatolian Black cattle. But Pit-1/HinfI gene and GHR/Alul polymorphisms were not found statistically significant in the specified periods, at all characters. On the other hand, since the MSTN/BstF5I gene was found to be monomorphic, no association analysis was performed between the measured values and this gene. In conclusion, mutation of these genes is difficult to suggest as a potential marker in a herd selection regarding the growth and development characteristics.

摘要

本研究的目的是确定安纳托利亚黑牛从出生到十二个月大的一些生长和发育特征与 Pit-1、GHR 和 Myostatin 基因之间的关系。PCR-RFLP方法用于检测多态性。发现基因型和等位基因频率为AA/AB/BB：0.096/0.519/0.385和A/B：0.356/0.644；Pit-1 和 GHR 基因中的 AA/AG/GG：0.346/0.385/0.269 和 A/G：0.538/0.462。发现 Myostatin 基因在所有基因型中也是单态的。^{虽然 Pit- 1}基因的卡方χ2检验显示符合 Hardy-Weinberg 平衡 ( p  > 0.05)，但在 GHR 基因中没有显示出一致性 ( p < 0.05)。统计分析结果表明，Pit-1和GHR基因多态性与安纳托利亚黑牛不同阶段年龄的生长性状之间存在关联。但 Pit-1/ Hinf I 基因和 GHR/ Alu l 多态性在特定时期、所有性状均无统计学意义。另一方面，由于发现MSTN/ BstF5 I基因是单态的，因此没有在测量值和该基因之间进行关联分析。总之，很难将这些基因的突变作为关于生长和发育特征的畜群选择中的潜在标志物。