Abstract

This study aims to determine the qualitative and quantitative contents of Turkish propolis collected from various regions of Turkey using high-performance liquid chromatography with the diode-array detector (HPLC-DAD) and gas chromatography-mass spectrometry (GC-MS) in water and ethanolic extracts. In HPLC-DAD analyses, it was determined that water extract of Turkish propolis contains phenolic acids such as caffeic acid (204.00 µg/mL), trans-cinnamic, chlorogenic, and caffeoylquinic acids, responsible for its antioxidant activity; whereas, the ethanolic extract of Turkish propolis contains chrysin (641.33 µg/mL), caffeic acid phenethyl ester (630.67 µg/mL), pinocembrin (572.67 µg/mL), galangin (534.11 µg/mL), naringenin (372.39 µg/mL), and also kaempferol, trans-cinnamic acid, caffeic acid, myricetin, and quercetin. GC-MS analyses showed that ethanolic extract of propolis contains caffeic acid by Rtx-1 column and the water extract of propolis contains quinic acid and ferulic acid by Rtx-5ms column. Various sugar derivatives were detected by both columns in water and ethanolic extracts of Turkish propolis. HPLC-DAD can be considered as a more effective method than GC-MS for the chemical characterization of propolis. Water extract of Turkish propolis can be a good source of raw materials for various sectors, as it is both cheap and has less health risk than ethanolic extract, and is suitable for human use.

摘要

这项研究的目的是使用二极管阵列检测器（HPLC-DAD）和气相色谱-质谱法（GC-MS）在高效液相色谱法中测定从土耳其各个地区收集的土耳其蜂胶的定性和定量含量。乙醇提取物。在HPLC-DAD分析中，确定土耳其蜂胶的水提取物含有酚酸，例如咖啡酸（204.00 µg / mL），反式肉桂酸，绿原酸和咖啡酰奎尼酸，这归因于其抗氧化活性。而土耳其蜂胶的乙醇提取物则包含了菊花蛋白（641.33 µg / mL），咖啡酸苯乙酯（630.67 µg / mL），匹诺贝林（572.67 µg / mL），高良姜精（534.11 µg / mL），柚皮苷（372.39 µg / mL）。 ），以及山emp酚，反肉桂酸，咖啡酸，杨梅素和槲皮素。GC-MS分析表明，Rtx-1色谱柱的蜂胶乙醇提取物含有咖啡酸，Rtx-5ms色谱柱的蜂胶水提取物含有奎尼酸和阿魏酸。土耳其蜂胶的水和乙醇提取物中的色谱柱均检测到各种糖衍生物。对于蜂胶的化学表征，HPLC-DAD被认为是比GC-MS更有效的方法。土耳其蜂胶的水提取物比乙醇提取物便宜而且对人体健康的危害小，因此可以用作各个部门的良好原材料来源，并且适合人类使用。土耳其蜂胶的水和乙醇提取物中的色谱柱均检测到各种糖衍生物。对于蜂胶的化学表征，HPLC-DAD被认为是比GC-MS更有效的方法。土耳其蜂胶的水提取物比乙醇提取物便宜而且对人体健康的危害小，因此可以用作各个部门的良好原材料来源，并且适合人类使用。土耳其蜂胶的水和乙醇提取物中的色谱柱均检测到各种糖衍生物。对于蜂胶的化学表征，HPLC-DAD被认为是比GC-MS更有效的方法。土耳其蜂胶的水提取物比乙醇提取物便宜而且对人体健康的危害小，因此可以用作各个部门的良好原材料来源，并且适合人类使用。